Use of dynamic light scattering and small-angle X-ray scattering to characterize new surfactants in solution conditions for membrane-protein crystallization

被引：6
作者：
Dahani, Mohamed [1 ]
Barret, Laurie-Anne [1 ,2 ]
Raynal, Simon [1 ]
Jungas, Colette [2 ]
Pernot, Petra [3 ]
Polidori, Ange [1 ]
Bonnete, Francoise [1 ]
机构：
[1] Avignon Univ, UMR 5247, CBSA, Inst Biomol Max Mousseron, F-84000 Avignon, France
[2] Lab Bioenerget Cellulaire Biol Vegetale & Microbi, UMR 7265, F-13108 St Paul Les Durance, France
[3] European Synchrotron Radiat Facil, F-38000 Grenoble, France
来源：
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2015年 / 71卷
关键词：
membrane protein; surfactant micelle; scattering techniques; phase diagram; crystallization; 2ND VIRIAL-COEFFICIENT; HEMIFLUORINATED SURFACTANTS; OMPF PORIN; FACIAL AMPHIPHILES; AQUEOUS-SOLUTIONS; DETERGENT; SOLUBILIZATION; STABILIZATION; SAXS; MACROMOLECULES;
D O I：
10.1107/S2053230X15009516
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The structural and interactive properties of two novel hemifluorinated surfactants, F2H9-beta-M and F4H5-beta-M, the syntheses of which were based on the structure and hydrophobicity of the well known dodecyl-beta-maltoside (DD-beta-M), are described. The shape of their micellar assemblies was characterized by small-angle X-ray scattering and their intermicellar inter-actions in crystallizing conditions were measured by dynamic light scattering. Such information is essential for surfactant phase-diagram determination and membrane-protein crystallization.
引用
收藏
页码：838 / 846
页数：9
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