Identification and Characterization of Cancer Stem Cells from Head and Neck Squamous Cell Carcinoma Cell Lines

被引：67

作者：

Pozzi, Valentina ^{[1
,2
]}

Sartini, Davide ^{[1
]}

Rocchetti, Romina ^{[3
]}

Santarelli, Andrea ^{[1
,4
]}

Rubini, Corrado ^{[3
]}

Morganti, Stefano ^{[1
]}

Giuliante, Rachela ^{[1
]}

Calabrese, Stefania ^{[1
]}

Di Ruscio, Giulia ^{[1
]}

Orlando, Fiorenza ^{[5
]}

Provinciali, Mauro ^{[6
]}

Saccucci, Franca ^{[1
]}

Lo Muzio, Lorenzo ^{[7
]}

Emanuelli, Monica ^{[1
,2
]}

机构：

[1] Polytech Univ Marche, Dept Clin Sci, I-60131 Ancona, Italy

[2] Polytech Univ Marche, New York Marche Struct Biol Ctr NY MaSBiC, I-60131 Ancona, Italy

[3] Polytech Univ Marche, Dept Biomed Sci & Publ Hlth, I-60131 Ancona, Italy

[4] IRCCS INRCA, Ancona, Italy

[5] IRCCS INRCA, Expt Anim Models Aging Units, Ancona, Italy

[6] IRCCS INRCA, Adv Technol Ctr Aging Res, Sci Technol Area, Ancona, Italy

[7] Univ Foggia, Dept Clin & Expt Med, Foggia, Italy

来源：

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY | 2015年 / 36卷 / 02期

关键词：

Head and neck squamous cell carcinoma; Cancer stem cells; Nicotinamide N-Methyltransferase; NICOTINAMIDE N-METHYLTRANSFERASE; ACUTE MYELOID-LEUKEMIA; HUMAN BRAIN-TUMORS; LUNG-CANCER; COLORECTAL-CANCER; EXPRESSION; MARKER; RESISTANCE; DIFFERENTIATION; SUBPOPULATION;

D O I：

10.1159/000430138

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Background/Aims: Head and neck squamous cell carcinoma (HNSCC) ranks sixth worldwide for tumor-related mortality. A subpopulation of tumor cells, termed cancer stem cells (CSCs), has the ability to support cancer growth. Therefore, profiling CSC-enriched populations could be a reliable tool to study cancer biology. Methods: We performed phenotypic characterization of 7 HNSCC cell lines and evaluated the presence of CSCs. CSCs from Hep-2 cell line and HNSCC primary cultures were enriched through sphere formation and sphere-forming cells have been characterized both in vitro and in vivo. In addition, we investigated the expression levels of Nicotinamide N-methyltransferase (NNMT), an enzyme overexpressed in several malignancies. Results: CSC markers were markedly expressed in Hep-2 cell line, which was found to be highly tumorigenic. CSC-enriched populations displayed increased expression of CSC markers and a strong capability to form tumors in vivo. We also found an overexpression of CSC markers in tumor formed by CSC-enriched populations. Interestingly, NNMT levels were significantly higher in CSC-enriched populations compared with parental cells. Conclusion: Our study provides an useful procedure for CSC identification and enrichment in HNSCC. Moreover, results obtained seem to suggest that CSCs may represent a promising target for an anticancer therapy. Copyright (C) 2015 S. Karger AG, Basel

引用

页码：784 / 798

页数：15

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