Novel reversed-phase high-performance liquid chromatography stationary phase with oligo(ethylene glycol) "click" to silica

被引：44
作者：
Guo, Zhimou [1 ]
Liu, Yanfang [1 ]
Xu, Junyan [1 ,2 ]
Xu, Qing [1 ]
Xue, Xingya [1 ]
Zhang, Feifang [1 ]
Ke, Yanxiong [1 ,2 ]
Liang, Xinmiao [1 ,2 ]
Lei, Aiwen [3 ]
机构：
[1] Chinese Acad Sci, Dalian Inst Chem Phys, Grad Sch, Dalian 116023, Peoples R China
[2] E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China
[3] Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China
来源：
JOURNAL OF CHROMATOGRAPHY A | 2008年 / 1191卷 / 1-2期
关键词：
oligo(ethylene glycol); stationary phase; click chemistry; high-performance liquid chromatography;
D O I：
10.1016/j.chroma.2007.11.093
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Oligo(ethylene glycol) (OEG) covalently bonded silica was prepared by using click chemistry and employed as a stationary phase for reversed-phase high-performance liquid chromatography. The column efficiency and effect of organic modifier content on retention were investigated. The separation selectivity was also studied with phenyl compounds and an actual sample of natural products. The results indicated that the stationary phase possessed good separation efficiency and separation selectivity in R-P-HPLC mode. Moreover, the stationary phase showed good complementary separation selectivity to the C18 stationary phase. The OEG stationary phase had "clustering" function for "homologous component" in the separation of natural products. (c) 2007 Elsevier B.V. All rights reserved.
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页码：78 / 82
页数：5
相关论文
共 37 条
[1]   Silica-bonded calixarenes in chromatography .1. Synthesis and characterization by solid-state NMR spectroscopy [J].
Brindle, R ;
Albert, K ;
Harris, SJ ;
Troltzsch, C ;
Horne, E ;
Glennon, JD .
JOURNAL OF CHROMATOGRAPHY A, 1996, 731 (1-2) :41-46
[2]   Comprehensive two-dimensional liquid chromatography with parallel gradients for separation of phenolic and flavone antioxidants [J].
Cacciola, F. ;
Jandera, P. ;
Hajdu, Z. ;
Cesla, P. ;
Mondello, L. .
JOURNAL OF CHROMATOGRAPHY A, 2007, 1149 (01) :73-87
[3]   New stationary phases for high-performance liquid chromatography based on poly(methyltetradecylsiloxane) thermally immobilized onto zirconized silica [J].
Faria, Anizio M. ;
Collins, Kenneth E. ;
Collins, Carol H. .
JOURNAL OF CHROMATOGRAPHY A, 2006, 1122 (1-2) :114-122
[4]   Poly(ethyleneglycol) column for the determination of acetaminophen, phenylephrine and chlorpheniramine in pharmaceutical formulations [J].
García, A ;
Rupérez, FJ ;
Marín, A ;
de la Maza, A ;
Barbas, C .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2003, 785 (02) :237-243
[5]   Silica-bonded calixarenes in chromatography .2. Chromatographic retention of metal ions and amino acid ester hydrochlorides [J].
Glennon, JD ;
Horne, E ;
Hall, K ;
Cocker, D ;
Kuhn, A ;
Harris, SJ ;
McKervey, MA .
JOURNAL OF CHROMATOGRAPHY A, 1996, 731 (1-2) :47-55
[6]   ENHANCED CHROMATOGRAPHIC SELECTIVITY FOR NA+ IONS ON A CALIXARENE-BONDED SILICA PHASE [J].
GLENNON, JD ;
OCONNOR, K ;
SRIJARANAI, S ;
MANLEY, K ;
HARIS, SJ ;
MCKERVEY, MA .
ANALYTICAL LETTERS, 1993, 26 (01) :153-162
[7]   Click chemistry: a new facile and efficient strategy for preparation of functionalized HPLC packings [J].
Guo, Zhimou ;
Lei, Aiwen ;
Liang, Xinmiao ;
Xu, Qing .
CHEMICAL COMMUNICATIONS, 2006, (43) :4512-4514
[8]   Molecular conformation in oligo(ethylene glycol)-terminated self-assembled monolayers on gold and silver surfaces determines their ability to resist protein adsorption [J].
Harder, P ;
Grunze, M ;
Dahint, R ;
Whitesides, GM ;
Laibinis, PE .
JOURNAL OF PHYSICAL CHEMISTRY B, 1998, 102 (02) :426-436
[9]  
Kolb HC, 2001, ANGEW CHEM INT EDIT, V40, P2004, DOI 10.1002/1521-3773(20010601)40:11<2004::AID-ANIE2004>3.0.CO
[10]  
2-5
1234