DNA binding and cleavage by a cationic manganese porphyrin-peptide nucleic acid conjugate

被引：48
作者：
Bigey, P
Sonnichsen, SH
Meunier, B
Nielsen, PE
机构：
[1] UNIV COPENHAGEN, PANUM INST, DEPT BIOCHEM B, CTR BIOMOL RECOGNIT, DK-2200 COPENHAGEN N, DENMARK
[2] CNRS, CHIM COORDINAT LAB, F-31077 TOULOUSE, FRANCE
来源：
BIOCONJUGATE CHEMISTRY | 1997年 / 8卷 / 03期
关键词：
D O I：
10.1021/bc9700190
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A cationic manganese porphyrin-peptide nucleic acid (PNA) conjugate has been prepared and used to cleave a double-stranded DNA target. Cleavage experiments were performed with a 247-base pair restriction DNA fragment containing a 10-base pair homopurine binding target for the PNA. Oxidative activation by this Mn porphyrin-PNA conjugate leads to sequence specific, 3'-staggered cleavage of both DNA strands near the strand displacement junction. Furthermore, the Mn porphyrin-PNA porphyrin conjugates bind over 100-fold better to double-stranded DNA compared to the native PNA.
引用
收藏
页码：267 / 270
页数：4
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