An Improved and High Throughput Respiratory Syncytial Virus (RSV) Micro-neutralization Assay

被引：5

作者：

Lien Anh Ha Do ^{[1
,2
]}

Tse, Reuben ^{[1
]}

Nathanielsz, Jordan ^{[1
]}

Anderson, Jeremy ^{[1
]}

Ong, Darren Suryawijaya ^{[1
]}

Chappell, Keith ^{[3
]}

Mulholland, Kim ^{[1
,2
,4
]}

Licciardi, Paul V. ^{[1
,2
]}

机构：

[1] Murdoch Childrens Res Inst, Parkville, Vic, Australia

[2] Univ Melbourne, Dept Paediat, Melbourne, Vic, Australia

[3] Univ Queensland, Sch Chem & Mol Biosci, Brisbane, Qld, Australia

[4] London Sch Hyg & Trop Med, London, England

来源：

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2019年 / 143期

基金：

澳大利亚国家健康与医学研究理事会;

关键词：

Medicine; Issue; 143; Respiratory syncytial virus; neutralizing antibody; antibody; plaque reduction; infectious disease; standardization; ANTIBODY;

D O I：

10.3791/59025

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Respiratory syncytial virus-specific neutralizing antibodies (RSV NAbs) are an important marker of protection against RSV. A number of different assay formats are currently in use worldwide so there is a need for an accurate and high-throughput method for measuring RSV NAbs. We describe here an imaging-based micro-neutralization assay that has been tested on RSV subgroup A and can also be adapted for RSV subgroup B and different sample types. This method is highly reproducible, with inter-assay variations for the reference antiserum being less than 10%. We believe this assay can be readily established in many laboratories worldwide at relatively low cost. Development of an improved, high-throughput assay that measures RSV NAbs represents a significant step forward for the standardization of this method internationally as well as being critical for the evaluation of novel RSV vaccine candidates in the future.

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页数：9

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