Physicochemical characterization and study of in vitro interactions of pH-sensitive liposomes with the complement system

被引:0
|
作者
Carmo, Vildete A. S. [1 ]
De Oliveira, Monica C. [1 ]
Reis, Eduardo C. O. [1 ]
Guimaraes, Tania M. P. D. [1 ]
Vilela, Jose M. C. [2 ]
Andrade, Margareth S. [2 ]
Michalick, Marilene S. M. [3 ]
Cardoso, Valbert N. [1 ]
机构
[1] Univ Fed Minas Gerais, Fac Farm, BR-31270901 Belo Horizonte, MG, Brazil
[2] Fdn Ctr Tecnol Minas Gerais, BR-31270901 Belo Horizonte, MG, Brazil
[3] Univ Fed Minas Gerais, Inst Ciencias Biol, BR-31270901 Belo Horizonte, MG, Brazil
关键词
pH-sensitive liposomes; DOPE; CHEMS; complement activation; hemolytic assay;
D O I
10.1080/08982100801893986
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Complement activation is an important step in the acceleration of liposome clearance. The anaphylatoxins released following complement activation may motivate a wide variety of physiologic changes. We performed physicochemical characterization and in vitro studies of the interaction of complement system with both noncirculating and long-circulating pH-sensitive and nonpH-sensitive liposomes. The liposomes were characterized by diameter, zeta potential, and atomic force microscopy (AFM). The study of liposome interactions with complement system was conducted using hemolytic assay in rat serum. All liposomes presented a similar mean diameter (between 99.8 and 124.3 nm). The zeta potential was negative in all liposome preparations, except in liposomes modified with aminopoly (ethyleneglycol) 2000-distearoylphosphatidylethanolamine (aPEG(2000)-DSPE), which presented positive zeta potential. Atomic force microscopy images showed that non-long-circulating pH-sensitive liposomes are prone to vesicles aggregation. Non-pH-sensitive liposomes complement system activates, while pH-sensitive liposomes showed to be poor complement activators in rat serum.
引用
收藏
页码:59 / 70
页数:12
相关论文
共 50 条
  • [1] VINORELBINE-LOADED pH-SENSITIVE LIPOSOMES: DEVELOPMENT, CHARACTERIZATION AND IN VITRO EVALUATION
    Toma, Ioana
    Raduly, Lajos
    Porfire, Alina
    Tefas, Lucia Ruxandra
    Zanoaga, Oana
    Berindan-Neagoe, Ioana
    Tomuta, Ioan
    FARMACIA, 2024, 72 (05) : 1162 - 1170
  • [2] Interactions between pH-sensitive liposomes and model membranes
    Bergstrand, N
    Arfvidsson, MC
    Kim, JM
    Thompson, DH
    Edwards, K
    BIOPHYSICAL CHEMISTRY, 2003, 104 (01) : 361 - 379
  • [3] Tunable pH-sensitive liposomes
    Hafez, IM
    Cullis, PR
    LIPOSOMES, PT D, 2004, 387 : 113 - 134
  • [4] pH-Sensitive Interactions between Cellulose Nanocrystals and DOPC Liposomes
    Navon, Yotam
    Radavidson, Harisoa
    Putaux, Jean-Luc
    Jean, Bruno
    Heux, Laurent
    BIOMACROMOLECULES, 2017, 18 (09) : 2918 - 2927
  • [5] TEMPERATURE-SENSITIVE AND PH-SENSITIVE LIPOSOMES
    OZER, AY
    FARIVAR, M
    HINCAL, AA
    EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, 1993, 39 (03) : 97 - 101
  • [6] MECHANISM OF LEAKAGE OF PH-SENSITIVE LIPOSOMES
    ELLENS, H
    BENTZ, J
    SZOKA, FC
    JOURNAL OF CELL BIOLOGY, 1983, 97 (05): : A109 - A109
  • [7] Sterically stabilized pH-sensitive liposomes
    Slepushkin, V
    Simoes, S
    De Lima, MCP
    Düzgünes, N
    LIPOSOMES, PT D, 2004, 387 : 134 - 147
  • [8] PH-SENSITIVE LIPOSOMES - INVITRO STUDIES
    YATVIN, MB
    CREE, TC
    TEGMOLARSSON, IM
    GIPP, JJ
    BIOPHYSICAL JOURNAL, 1982, 37 (02) : A211 - A211
  • [9] PH-sensitive liposomes in drug delivery
    Paliwal, Shivani Rai
    Paliwal, Rishi
    Vyas, Suresh P.
    RSC Smart Materials, 2013, 1 (01): : 80 - 93
  • [10] Preparation and investigation of in vitro cytotoxic activity of pH-sensitive liposomes with sanguinarine
    Lutsenko, S., V
    Gudkova, O., I
    Kashirin, V. V.
    Gromovykh, T., I
    Feldman, N. B.
    INTERNATIONAL JOURNAL OF NANOTECHNOLOGY, 2019, 16 (1-3) : 77 - 86