Effect of Hg2+ on HydSL Hydrogenase of the Purple Sulfur Bacteria Thiocapsa roseopersicina BBS

被引：2
作者：
Zorin, N. A. [1 ]
Starodubov, A. S. [1 ]
Tsygankov, A. A. [1 ]
机构：
[1] Russian Acad Sci, Separate Div Fed State Budgetary Inst, Pushchino Res Ctr Biol Res, Sci Fed Res Ctr,Inst Basic Biol Problems, Pushchino 142290, Moscow Oblast, Russia
来源：
APPLIED BIOCHEMISTRY AND MICROBIOLOGY | 2020年 / 56卷 / 02期
关键词：
mercury(II) chloride; hydrogen; hydrogenase; infrared spectra; NiFe active center; iron-sulfur clusters; INHIBITION; METABOLISM;
D O I：
10.1134/S0003683820020155
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The inhibitory effect of mercury(II) chloride on the activity and structure of the hydrogenase Thiocapsa roseopersicina BBS was studied. The kinetics of hydrogenase inactivation in the presence of different inhibitor concentrations was determined. The irreversible nature of Hg2+ action was established, and hydrogenase inhibition constants at different temperatures were determined. The presence of this inhibitor in enzyme solution significantly reduced its stability and caused denaturation at temperatures above 50 degrees C. In the process of enzyme incubation with Hg2+, the absorption band fades in the visible region of the spectrum, indicating the destruction of iron-sulfur clusters. Comparative analysis of the infrared Fourier spectra of hydrogenase without the addition and after incubation with inhibitor indicates the destruction of the NiFe active center.
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页码：149 / 153
页数：5
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