Hesperedin promotes MyoD-induced myogenic differentiation in vitro and in vivo
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作者:
Jeong, Hana
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Ewha Womans Univ, Coll Pharm, Seoul 120750, South Korea
Ewha Womans Univ, Div Life & Pharmaceut Sci, Seoul 120750, South Korea
Ewha Womans Univ, Ctr Cell Signaling & Drug Discovery Res, Seoul 120750, South KoreaEwha Womans Univ, Coll Pharm, Seoul 120750, South Korea
Jeong, Hana
[1
,2
,3
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Lee, Joo Yeon
[1
,2
,3
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Jang, Eun Jung
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机构:
Ewha Womans Univ, Coll Pharm, Seoul 120750, South Korea
Ewha Womans Univ, Div Life & Pharmaceut Sci, Seoul 120750, South Korea
Ewha Womans Univ, Ctr Cell Signaling & Drug Discovery Res, Seoul 120750, South KoreaEwha Womans Univ, Coll Pharm, Seoul 120750, South Korea
Jang, Eun Jung
[1
,2
,3
]
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Lee, Eun Hye
[1
,2
,3
]
Bae, Myung Ae
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机构:
Korea Res Inst Chem Technol, Taejon 305606, South KoreaEwha Womans Univ, Coll Pharm, Seoul 120750, South Korea
Bae, Myung Ae
[4
]
Hong, Jeong-Ho
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Korea Univ, Sch Life Sci & Biotechnol, Seoul, South KoreaEwha Womans Univ, Coll Pharm, Seoul 120750, South Korea
Hong, Jeong-Ho
[5
]
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Hwang, Eun Sook
[1
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,3
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机构:
[1] Ewha Womans Univ, Coll Pharm, Seoul 120750, South Korea
[2] Ewha Womans Univ, Div Life & Pharmaceut Sci, Seoul 120750, South Korea
[3] Ewha Womans Univ, Ctr Cell Signaling & Drug Discovery Res, Seoul 120750, South Korea
[4] Korea Res Inst Chem Technol, Taejon 305606, South Korea
[5] Korea Univ, Sch Life Sci & Biotechnol, Seoul, South Korea
BACKGROUND AND PURPOSE The bioflavonoid, hesperedin, promotes osteoblast differentiation in human mesenchymal stem cells, indicating an anabolic effect of hesperedin on bone metabolism. Murine bone marrow mesenchymal stem cells undergo myogenic differentiation as well as osteogenic differentiation. We therefore explored whether hesperedin modulates muscle cell differentiation. EXPERIMENTAL APPROACH Myoblast C2C12 cells were differentiated into muscle cells in the presence or absence of hesperedin. The effects of hesperedin on myogenic differentiation were determined by analysing specific muscle markers in vitro using reporter gene assays, immunoblotting, RT-PCR and DNA pull-down assays. In vivo, the effects of hesperedin were assessed using the freeze injury-induced muscle regeneration model in mice and daily injections of hesperedin for 6 days. KEY RESULTS Hesperedin promoted myogenic differentiation, in a dose-dependent manner, by increasing myogenin gene expression. MyoD-induced myogenin gene transcription was enhanced by hesperedin, as this bioflavonoid augmented the nuclear localization and myogenin promoter-binding of MyoD. In addition, hesperedin increased myogenin and muscle creatine kinase gene expression during myogenic differentiation from C3H10T1/2 mesenchymal stem cells in a MyoD-dependent manner and accelerated in vivo muscle regeneration induced by muscle injury. CONCLUSIONS AND IMPLICATIONS Our results demonstrate that hesperedin promoted myogenic differentiation in vitro and in vivo through activation of MyoD-mediated myogenin expression, suggesting a beneficial role in promoting muscle regeneration, following injury.
机构:
Chinese Univ Hong Kong, Dept Orthopaed & Traumatol, Li Ka Shing Inst Hlth Sci, Hong Kong, Peoples R ChinaChinese Univ Hong Kong, Dept Orthopaed & Traumatol, Li Ka Shing Inst Hlth Sci, Hong Kong, Peoples R China
Zhao, Yu
Zhou, Jiajian
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Chinese Univ Hong Kong, Dept Chem Pathol, Li Ka Shing Inst Hlth Sci, Hong Kong, Peoples R China
Southern Med Univ, Dermatol Hosp, Guangzhou, Peoples R ChinaChinese Univ Hong Kong, Dept Orthopaed & Traumatol, Li Ka Shing Inst Hlth Sci, Hong Kong, Peoples R China
Zhou, Jiajian
He, Liangqiang
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Chinese Univ Hong Kong, Dept Chem Pathol, Li Ka Shing Inst Hlth Sci, Hong Kong, Peoples R ChinaChinese Univ Hong Kong, Dept Orthopaed & Traumatol, Li Ka Shing Inst Hlth Sci, Hong Kong, Peoples R China
He, Liangqiang
Li, Yuying
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Chinese Univ Hong Kong, Dept Chem Pathol, Li Ka Shing Inst Hlth Sci, Hong Kong, Peoples R ChinaChinese Univ Hong Kong, Dept Orthopaed & Traumatol, Li Ka Shing Inst Hlth Sci, Hong Kong, Peoples R China
Li, Yuying
Yuan, Jie
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Chinese Univ Hong Kong, Dept Chem Pathol, Li Ka Shing Inst Hlth Sci, Hong Kong, Peoples R ChinaChinese Univ Hong Kong, Dept Orthopaed & Traumatol, Li Ka Shing Inst Hlth Sci, Hong Kong, Peoples R China
Yuan, Jie
Sun, Kun
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机构:
Chinese Univ Hong Kong, Dept Chem Pathol, Li Ka Shing Inst Hlth Sci, Hong Kong, Peoples R China
Shenzhen Bay Lab, Inst Canc Res, Shenzhen 518000, Peoples R ChinaChinese Univ Hong Kong, Dept Orthopaed & Traumatol, Li Ka Shing Inst Hlth Sci, Hong Kong, Peoples R China
Sun, Kun
Chen, Xiaona
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Chinese Univ Hong Kong, Dept Orthopaed & Traumatol, Li Ka Shing Inst Hlth Sci, Hong Kong, Peoples R ChinaChinese Univ Hong Kong, Dept Orthopaed & Traumatol, Li Ka Shing Inst Hlth Sci, Hong Kong, Peoples R China
Chen, Xiaona
Bao, Xichen
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机构:
Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Lab RNA Mol Biol, Guangzhou 510530, Peoples R ChinaChinese Univ Hong Kong, Dept Orthopaed & Traumatol, Li Ka Shing Inst Hlth Sci, Hong Kong, Peoples R China
Bao, Xichen
Esteban, Miguel A.
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机构:
Chinese Acad Sci, South China Inst Stem Cell Biol & Regenerat Med, Guangzhou Inst Biomed & Hlth, Lab Chromatin & Human Dis,Key Lab Regenerat Biol, Guangzhou, Peoples R ChinaChinese Univ Hong Kong, Dept Orthopaed & Traumatol, Li Ka Shing Inst Hlth Sci, Hong Kong, Peoples R China
Esteban, Miguel A.
Sun, Hao
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Chinese Univ Hong Kong, Dept Chem Pathol, Li Ka Shing Inst Hlth Sci, Hong Kong, Peoples R ChinaChinese Univ Hong Kong, Dept Orthopaed & Traumatol, Li Ka Shing Inst Hlth Sci, Hong Kong, Peoples R China
Sun, Hao
Wang, Huating
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Chinese Univ Hong Kong, Dept Orthopaed & Traumatol, Li Ka Shing Inst Hlth Sci, Hong Kong, Peoples R ChinaChinese Univ Hong Kong, Dept Orthopaed & Traumatol, Li Ka Shing Inst Hlth Sci, Hong Kong, Peoples R China
机构:Korea Univ, Coll Life Sci & Biotechnol, Div Biotechnol, Seoul 136701, South Korea
Jin, Xun
Kim, Jong-Gun
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机构:Korea Univ, Coll Life Sci & Biotechnol, Div Biotechnol, Seoul 136701, South Korea
Kim, Jong-Gun
Oh, Myung-Joo
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机构:Korea Univ, Coll Life Sci & Biotechnol, Div Biotechnol, Seoul 136701, South Korea
Oh, Myung-Joo
Oh, Ho-Yeon
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机构:Korea Univ, Coll Life Sci & Biotechnol, Div Biotechnol, Seoul 136701, South Korea
Oh, Ho-Yeon
Sohn, Young-Woo
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机构:Korea Univ, Coll Life Sci & Biotechnol, Div Biotechnol, Seoul 136701, South Korea
Sohn, Young-Woo
Pian, Xumin
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机构:Korea Univ, Coll Life Sci & Biotechnol, Div Biotechnol, Seoul 136701, South Korea
Pian, Xumin
Yin, Jin Long
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机构:Korea Univ, Coll Life Sci & Biotechnol, Div Biotechnol, Seoul 136701, South Korea
Yin, Jin Long
Beck, Samuel
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机构:Korea Univ, Coll Life Sci & Biotechnol, Div Biotechnol, Seoul 136701, South Korea
Beck, Samuel
Lee, Namkyung
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机构:Korea Univ, Coll Life Sci & Biotechnol, Div Biotechnol, Seoul 136701, South Korea
Lee, Namkyung
Son, Jeesoo
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机构:Korea Univ, Coll Life Sci & Biotechnol, Div Biotechnol, Seoul 136701, South Korea
Son, Jeesoo
Kim, Hyunggee
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机构:Korea Univ, Coll Life Sci & Biotechnol, Div Biotechnol, Seoul 136701, South Korea
Kim, Hyunggee
Yan, Changguo
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机构:Korea Univ, Coll Life Sci & Biotechnol, Div Biotechnol, Seoul 136701, South Korea
Yan, Changguo
Wang, J. Hui
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机构:Korea Univ, Coll Life Sci & Biotechnol, Div Biotechnol, Seoul 136701, South Korea
Wang, J. Hui
Choi, Yun-Jaie
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机构:Korea Univ, Coll Life Sci & Biotechnol, Div Biotechnol, Seoul 136701, South Korea
Choi, Yun-Jaie
Whang, Kwang Youn
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Korea Univ, Coll Life Sci & Biotechnol, Div Biotechnol, Seoul 136701, South KoreaKorea Univ, Coll Life Sci & Biotechnol, Div Biotechnol, Seoul 136701, South Korea
机构:
Sookmyung Womens Univ, Coll Pharm, Res Ctr Cell Fate Control, Seoul, South KoreaSookmyung Womens Univ, Coll Pharm, Res Ctr Cell Fate Control, Seoul, South Korea
Lee, Sang-Jin
Leem, Young-Eun
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Sungkyunkwan Univ, Dept Mol Cell Biol, Single Cell Network Res Ctr, Sch Med, Suwon, South KoreaSookmyung Womens Univ, Coll Pharm, Res Ctr Cell Fate Control, Seoul, South Korea
Leem, Young-Eun
Go, Ga-Yeon
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Sookmyung Womens Univ, Coll Pharm, Res Ctr Cell Fate Control, Seoul, South KoreaSookmyung Womens Univ, Coll Pharm, Res Ctr Cell Fate Control, Seoul, South Korea
Go, Ga-Yeon
Choi, Younhee
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Sookmyung Womens Univ, Coll Pharm, Res Ctr Cell Fate Control, Seoul, South KoreaSookmyung Womens Univ, Coll Pharm, Res Ctr Cell Fate Control, Seoul, South Korea
Choi, Younhee
Song, Yoo Jin
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Sookmyung Womens Univ, Coll Pharm, Res Ctr Cell Fate Control, Seoul, South KoreaSookmyung Womens Univ, Coll Pharm, Res Ctr Cell Fate Control, Seoul, South Korea
Song, Yoo Jin
Kim, Insol
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Sookmyung Womens Univ, Coll Pharm, Res Ctr Cell Fate Control, Seoul, South KoreaSookmyung Womens Univ, Coll Pharm, Res Ctr Cell Fate Control, Seoul, South Korea
Kim, Insol
Kim, Do Yoon
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Sookmyung Womens Univ, Coll Pharm, Res Ctr Cell Fate Control, Seoul, South KoreaSookmyung Womens Univ, Coll Pharm, Res Ctr Cell Fate Control, Seoul, South Korea
Kim, Do Yoon
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Kim, Yong Kee
Seo, Dong-Wan
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Dankook Univ, Coll Pharm, Cheonan, South KoreaSookmyung Womens Univ, Coll Pharm, Res Ctr Cell Fate Control, Seoul, South Korea
Seo, Dong-Wan
Kang, Jong-Sun
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Sungkyunkwan Univ, Dept Mol Cell Biol, Single Cell Network Res Ctr, Sch Med, Suwon, South KoreaSookmyung Womens Univ, Coll Pharm, Res Ctr Cell Fate Control, Seoul, South Korea