Isolation and characterization of tri and tetranucleotide microsatellite markers for the tambaqui (Colossoma macropomum, Serrasalmidae, Characiformes)

被引：12

作者：

Hamoy, Igor Guerreiro ^{[1
]}

Cidade, Fernanda Witt ^{[2
]}

Barbosa, Maria Silvanira ^{[3
]}

Goncalves, Evonnildo Costa ^{[3
]}

Santos, Sidney ^{[1
]}

机构：

[1] Fed Univ Para, Inst Ciencias Biol, Lab Genet Humana & Med, BR-66059 Belem, Para, Brazil

[2] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, Campinas, SP, Brazil

[3] Fed Univ Para, Inst Ciencias Biol, Lab Polimorfismo DNA, BR-66059 Belem, Para, Brazil

来源：

CONSERVATION GENETICS RESOURCES | 2011年 / 3卷 / 01期

关键词：

Colossoma macropomum; Tambaqui; Microsatellite; Tri and tetranucleotide; GENETIC-VARIABILITY; SOFTWARE; AMAZON;

D O I：

10.1007/s12686-010-9275-5

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

We isolated 13 tri and tetranucleotide microsatellite markers for the species Colossoma macropomum that can be used in management programmes for this species of Amazon fish. This panel of microsatellite markers was used in the genotyping of 20 individual specimens collected in the lakes of Obidos city, in the Brazilian Amazon. The number of alleles per locus varied from four to ten. The observed heterozygosity varied from 0.31 to 0.95. We observed no significant deviation from the expected Hardy-Weinberg equilibrium assumption. In the sample investigated, it was not possible to identify any significant linkage disequilibrium among the 78 possible loci pairs. In our analysis, we found no indication of genotyping error attributed to stutter bands, large allele dropout or null alleles.

引用

页码：33 / 36

页数：4

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