Deinococcus depolymerans sp nov., a gamm- and UV-radiation-resistant bacterium, isolated from a naturally radioactive site

被引：27
作者：
Asker, Dalal [1 ,2 ,3 ]
Awad, Tarek S. [3 ]
McLandsborough, Lynne [3 ]
Beppu, Teruhiko [1 ]
Ueda, Kenji [1 ]
机构：
[1] Nihon Univ, Life Sci Res Ctr, Coll Bioresource Sci, Fujisawa, Kanagawa 2528510, Japan
[2] Univ Alexandria, Fac Agr, Dept Food Sci & Technol, Alexandria 21545, Egypt
[3] Univ Massachusetts, Dept Food Sci, Amherst, MA 01003 USA
来源：
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY | 2011年 / 61卷
基金：
日本学术振兴会;
关键词：
GENUS DEINOCOCCUS; JAPAN; DIVERSITY; DNA; MEMBERS; MISASA; DESERT; WATER;
D O I：
10.1099/ijs.0.013482-0
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Four gamma- and UV-radiation-resistant bacterial strains, designated TDMA-24(T), TDMA-24-2, TDMA-24-3 and TDMA-24-4, were isolated from a fresh-water sample collected at Misasa, Tottori, Japan. Cells of these strains were Gram-reaction-positive, non-motile, non-spore-forming, rod-shaped and formed red colonies. The genomic DNA G+C contents ranged from 70.5 to 70.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel isolates belong to the genus Deinococcus, the highest sequence similarities being with Deinococcus aquaticus PB314(T) (98 WO and Deinococcus caeni Ho-08(T) (97 %). The polar lipid profile of strain TDMA-24(T) comprised three unidentified phosphoglycolipids, five unidentified glycolipids and seven unidentified polar lipids. MK-8 was the predominant respiratory quinone. Major fatty acids were iso-C(15:0), C(15:1)omega 6c, C(15:0), C(16:0) and summed feature 3 (iso-C(15:0) 2-OH and/or C(16:1)omega 7c). On the basis of their phylogenetic positions and chemotaxonomic and phenotypic characteristics, the novel isolates represent a novel species of the genus Deinococcus, for which the name Deinococcus depolymerans sp. nov. is proposed. The type strain is TDMA-24(T) (=JCM 14369(T) =NBRC 102115(T) =CCUG 53609(T)).
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页码：1448 / 1453
页数：6
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