DNA isolation from museum and type collection slides of microsporidia

被引：18

作者：

Hylis, M ^{[1
]}

Weiser, J

Oborník, M

Vávra, J

机构：

[1] Charles Univ, Fac Sci, Dept Parasitol, Prague, Czech Republic

[2] Charles Univ, Fac Sci, Electron Microscopy Lab, Prague, Czech Republic

[3] Acad Sci Czech Republ, Inst Entomol, CR-37005 Ceske Budejovice, Czech Republic

[4] Acad Sci Czech Republ, Inst Parasitol, CR-37005 Ceske Budejovice, Czech Republic

来源：

JOURNAL OF INVERTEBRATE PATHOLOGY | 2005年 / 88卷 / 03期

关键词：

microsporidia; Lepidoptera; Trichoptera; DNA isolation; type collection; phylogeny;

D O I：

10.1016/j.jip.2005.02.004

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

DNA from 19 species of microsporidia was isolated and amplified from infected host tissue that were originally prepared between the years 1946 and 1996. The smears, on glass microscope slides, were either Giemsa-stained or unstained. Methanol-fixed, Giemsastained smears proved to be suitable for DNA isolation; DNA was amplified from only two of 14 unstained slides. The isolated DNA was successfully amplified in PCRs using small subunit and large subunit rDNA primers and sequenced. The high efficiency of DNA isolation demonstrates the usefulness of archival and type collection slides for some molecular biology and molecular taxonomy studies. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：257 / 260

页数：4