Liquid chromatography of spiramycin on poly(styrene-divinylbenzene)

被引：24

作者：

Liu, L ^{[1
]}

Roets, E ^{[1
]}

Hoogmartens, J ^{[1
]}

机构：

[1] KATHOLIEKE UNIV LEUVEN,FAC FARMACEUT WETENSCHAPPEN,LAB FARMACEUT CHEM & ANAL GENEESMIDDELEN,B-3000 LOUVAIN,BELGIUM

来源：

JOURNAL OF CHROMATOGRAPHY A | 1997年 / 764卷 / 01期

关键词：

mobile phase composition; pharmaceutical analysis; stationary phases; LC; spiramycin; antibiotics;

D O I：

10.1016/S0021-9673(96)00876-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An isocratic liquid chromatographic method is described using as stationary phase the very stable poly(styrene-divinylbenzene) PLRP-S (8 mu m 1000 Angstrom). The mobile phase is acetonitrile-0.2 M potassium phosphate buffer pH 9.0-water (37:5:up to 100, v/v/v), delivered at a flow-rate of 1.0 ml/min. UV detection is performed at 232 nm. Full factorial design was applied to evaluate the robustness of the method. Quantitative results of a number of commercial samples are presented.

引用

页码：43 / 53

页数：11

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