Global changes in phospholipids identified by MALDI MS in rats with focal cerebral ischemia

被引：60

作者：

Shanta, Selina Rahman ^{[1
]}

Choi, Chang Soon ^{[2
]}

Lee, Jeong Hwa ^{[1
]}

Shin, Chan Young ^{[2
]}

Kim, Young Jun ^{[3
]}

Kim, Kyun-Hwan ^{[4
,5
]}

Kim, Kwang Pyo ^{[1
]}

机构：

[1] Konkuk Univ, Dept Mol Biotechnol, WCU Program, Seoul 143701, South Korea

[2] Konkuk Univ, Dept Pharmacol, Sch Med, Seoul 143701, South Korea

[3] Konkuk Univ, Dept Appl Biochem, Chungju 380701, Chungbuk, South Korea

[4] Konkuk Univ, Sch Med, Dept Pharmacol, Seoul 143701, South Korea

[5] Konkuk Univ, Sch Med, Ctr Canc Res & Diagnost Med, IBST, Seoul 143701, South Korea

来源：

JOURNAL OF LIPID RESEARCH | 2012年 / 53卷 / 09期

基金：

新加坡国家研究基金会;

关键词：

brain imaging; matrix-assisted laser desorption/ionization; mass spectrometry; IMAGING MASS-SPECTROMETRY; ASSISTED LASER-DESORPTION; BRAIN-INJURY; LIPIDOMICS; MODELS; GENERATION; APOPTOSIS; CERAMIDE; STROKE; LIPIDS;

D O I：

10.1194/jlr.M022558

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Neuronal membrane phospholipids are highly affected by oxidative stress caused by ischemic injury. Thus, it is necessary to identify key lipid components that show changes during ischemia to develop an effective approach to prevent brain damage from ischemic injury. The recent development of MALDI imaging MS (MALDI IMS) makes it possible to identify phospholipids that change between damaged and normal regions directly from tissues. In this study, we conducted IMS on rat brains damaged by ischemic injury and detected various phospholipids that showed unique distributions between normal and damaged areas of the brain. Among them, we confirmed changes in phospholipids such as lysophosphatidylcholine, phosphatidylcholine, phosphatidylethanolamine, and sphingomyelin by MALDI IMS followed by MS/MS analysis. These lipids were present in high concentrations in the brain and are important for maintenance of cellular structure as well as production of second messengers for cellular signal transduction. Our results emphasize the identification of phospholipid markers for ischemic injury and successfully identified several distinctly located phospholipids in ischemic brain tissue.-Shanta, S. R., C. S. Choi, J. H. Lee, C. Y. Shin, Y. J. Kim, K-H. Kim, and K. P. Kim. Global changes in phospholipids identified by MALDI MS in rats with focal cerebral ischemia. J. Lipid Res. 2012. 53: 1823-1831.

引用

页码：1823 / 1831

页数：9

共 33 条

[1] RETRACTED: Secretory phospholipase A2 IIA is up-regulated by TNF-α and IL-1α/β after transient focal cerebral ischemia in rat (Retracted article. See vol. 1507, pg. 154, 2013) [J].

Adibhatla, Rao Muralikrishna ;

Hatcher, J. F. .

BRAIN RESEARCH, 2007, 1134 (01) :199-205

[2]

Adibhatla RM, 2006, AAPS J, V8, pE314

[3] Phospholipase A2, reactive oxygen species, and lipid peroxidation in cerebral ischemia [J].

Adibhatla, RM ;

Hatcher, JF .

FREE RADICAL BIOLOGY AND MEDICINE, 2006, 40 (03) :376-387

[4] Imaging mass spectrometry: Principles and potentials [J].

Chaurand, P ;

Schwartz, SA ;

Reyzer, ML ;

Caprioli, RA .

TOXICOLOGIC PATHOLOGY, 2005, 33 (01) :92-101

[5] Mass Spectrometric Imaging for Biomedical Tissue Analysis [J].

Chughtai, Kamila ;

Heeren, Ron M. A. .

CHEMICAL REVIEWS, 2010, 110 (05) :3237-3277

[6]

Drgová A, 2004, GEN PHYSIOL BIOPHYS, V23, P337

[7] Free fatty acids act as endogenous ionophores, resulting in Na+ and Ca2+ influx and myocyte apoptosis [J].

Fang, Kwang-Ming ;

Lee, An-Sheng ;

Su, Ming-Jai ;

Lin, Chien-Liang ;

Chien, Chung-Liang ;

Wu, Mei-Lin .

CARDIOVASCULAR RESEARCH, 2008, 78 (03) :533-545

[8]

Farooqui AA, 1997, J NEUROCHEM, V69, P889

[9] Glycerophospholipids in brain: their metabolism, incorporation into membranes, functions, and involvement in neurological disorders [J].

Farooqui, AA ;

Horrocks, LA ;

Farooqui, T .

CHEMISTRY AND PHYSICS OF LIPIDS, 2000, 106 (01) :1-29

[10] Imaging mass spectrometry for lipidomics [J].

Goto-Inoue, Naoko ;

Hayasaka, Takahiro ;

Zaima, Nobuhiro ;

Setou, Mitsutoshi .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 2011, 1811 (11) :961-969

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