High-Throughput PCR Assays To Monitor Wolbachia Infection in the Dengue Mosquito (Aedes aegypti) and Drosophila simulans

被引：82
作者：
Lee, Siu F. [1 ]
White, Vanessa L. [1 ]
Weeks, Andrew R. [1 ]
Hoffmann, Ary A. [1 ]
Endersby, Nancy M. [1 ]
机构：
[1] Univ Melbourne, Dept Genet, Inst Bio21, Melbourne, Vic 3010, Australia
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2012年 / 78卷 / 13期
基金：
澳大利亚研究理事会; 美国国家卫生研究院;
关键词：
CYTOPLASMIC INCOMPATIBILITY; POPULATIONS; MELANOGASTER; PHYLOGENY; EVOLUTION; SYMBIONT; INSECTS; CLONING;
D O I：
10.1128/AEM.00069-12
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We have developed and validated two new fluorescence-based PCR assays to detect the Wolbachia wMel strain in Aedes aegypti and the wRi and wAu strains in Drosophila simulans. The new assays are accurate, informative, and cost-efficient for large-scale Wolbachia screening.
引用
收藏
页码：4740 / 4743
页数：4
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