Role of the tumor necrosis factor-α, cyclooxygenase-2, prostaglandin E2, and effect of low-intensity pulsed ultrasound in an in vitro herniated disc resorption model

被引:20
|
作者
Iwabuchi, Sadahiro [1 ]
Ito, Masaya [2 ]
Chikanishi, Toshihiro [3 ,4 ]
Azuma, Yoshiaki [3 ,4 ]
Haro, Hirotaka
机构
[1] Teijin Pharma Ltd, Biomed Engn Labs, Tokyo 1918512, Japan
[2] Teijin Ltd, Dept Tissue Engn Dev, Innovat Res Inst, Tokyo, Japan
[3] Teijin Pharma Ltd, Teijin Inst Biomed Res, Tokyo, Japan
[4] Univ Yamanashi, Dept Orthopaed Surg, Yamanashi, Japan
关键词
low-intensity pulsed ultrasound; herniated disc; TNF-alpha; COX-2; PGE2;
D O I
10.1002/jor.20525
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
摘要
Spontaneous herniated disc resorption occurs via inflammatory reactions involving abundant neovascularization and macrophage phagocytotic activity. Nonthermal low-intensity pulsed ultrasound (LIPUS) treatment might be effective in shortening the duration of disc resorption. We developed a rat in vitro resorption model in which a coccygeal intervertebral disc and peritoneal macrophages were cocultured. Secretion of tumor necrosis factor-alpha (TNF-alpha) from macrophages was promoted by LIPUS, and the process of disc degeneration was thus accelerated. In this study, we further examined the effects of LIPUS using this in vitro model focusing on whether LIPUS affects cyclooxygenase-2 (COX-2) signaling pathways. We found that the levels of COX-2 and prostaglandin E2 (PGE2) secreted from macrophages were increased by LIPUS. However, these phenomena were not caused by LIPUS directly, as the levels of these substances were reduced by neutralizing TNF-alpha activity. Moreover, the wet weights of the disc samples were not changed by addition of PGE2, but were reduced by recombinant TNF-alpha. Our results suggest that the effects of LIPUS in enhancing the process of herniated disc resorption are caused mainly by TNF-alpha. (C) 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.
引用
收藏
页码:1274 / 1278
页数:5
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