A Bacterial Acetyltransferase Destroys Plant Microtubule Networks and Blocks Secretion

被引:140
作者
Lee, Amy Huei-Yi [1 ,2 ]
Hurley, Brenden [1 ]
Felsensteiner, Corinna [2 ]
Yea, Carmen [1 ]
Ckurshumova, Wenzislava [1 ]
Bartetzko, Verena [3 ]
Wang, Pauline W. [2 ]
Van Quach [1 ]
Lewis, Jennifer D. [1 ]
Liu, Yulu C. [4 ]
Boernke, Frederik [3 ]
Angers, Stephane [4 ,5 ]
Wilde, Andrew [5 ,6 ]
Guttman, David S. [1 ,2 ]
Desveaux, Darrell [1 ,2 ]
机构
[1] Univ Toronto, Dept Cell & Syst Biol, Toronto, ON, Canada
[2] Univ Toronto, Ctr Anal Genome Evolut & Funct, Toronto, ON, Canada
[3] Univ Erlangen Nurnberg, Inst Biol, Lehrstuhl Biochem, Erlangen, Germany
[4] Univ Toronto, Leslie Dan Fac Pharm, Toronto, ON, Canada
[5] Univ Toronto, Dept Biochem, Toronto, ON, Canada
[6] Univ Toronto, Dept Mol Genet, Toronto, ON, Canada
基金
加拿大健康研究院; 加拿大自然科学与工程研究理事会;
关键词
PATTERN-RECOGNITION RECEPTORS; PAMP-TRIGGERED IMMUNITY; III EFFECTOR HOPZ1; PSEUDOMONAS-SYRINGAE; CELL-WALL; INNATE IMMUNITY; HYPERSENSITIVE RESPONSE; GAMMA-TUBULIN; KAPPA-B; PROTEIN;
D O I
10.1371/journal.ppat.1002523
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The eukaryotic cytoskeleton is essential for structural support and intracellular transport, and is therefore a common target of animal pathogens. However, no phytopathogenic effector has yet been demonstrated to specifically target the plant cytoskeleton. Here we show that the Pseudomonas syringae type III secreted effector HopZ1a interacts with tubulin and polymerized microtubules. We demonstrate that HopZ1a is an acetyltransferase activated by the eukaryotic co-factor phytic acid. Activated HopZ1a acetylates itself and tubulin. The conserved autoacetylation site of the YopJ / HopZ superfamily, K289, plays a critical role in both the avirulence and virulence function of HopZ1a. Furthermore, HopZ1a requires its acetyltransferase activity to cause a dramatic decrease in Arabidopsis thaliana microtubule networks, disrupt the plant secretory pathway and suppress cell wall-mediated defense. Together, this study supports the hypothesis that HopZ1a promotes virulence through cytoskeletal and secretory disruption.
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页数:14
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