Inactivation and fragmentation of lectin from Bothrops leucurus snake venom by gamma irradiation

被引：4
|
作者：
Nunes, E. S. [1 ]
Souza, M. A. A. [1 ]
Vaz, A. F. M. [1 ]
Coelho, L. C. B. B. [1 ]
Aguiar, J. S. [2 ]
Silva, T. G. [2 ]
Guarnieri, M. C. [3 ]
Melo, A. M. M. A. [4 ]
Oliva, M. L. V. [5 ]
Correia, M. T. S. [1 ]
机构：
[1] Univ Fed Pernambuco, Dept Bioquim, BR-50670420 Recife, PE, Brazil
[2] Univ Fed Pernambuco, Dept Antibiot, BR-50670420 Recife, PE, Brazil
[3] Univ Fed Pernambuco, Dept Zool, BR-50670420 Recife, PE, Brazil
[4] Univ Fed Pernambuco, Dept Biofis & Radiobiol, BR-50670420 Recife, PE, Brazil
[5] Univ Fed Sao Paulo, Dept Bioquim, BR-04044020 Sao Paulo, Brazil
来源：
RADIATION PHYSICS AND CHEMISTRY | 2012年 / 81卷 / 04期
关键词：
Bothrops leucurus; Lectin; Gamma rays; Snake venom; PROTEINS;
D O I：
10.1016/j.radphyschem.2011.12.048
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
Gamma radiation alters the molecular structure of biomolecules and is able to mitigate the action of snake venoms and their isolated toxins. The effect of gamma-radiation on the folding of Bothrops lecurus venom lectin was measured by a hemagglutinating assay, intrinsic and bis-ANS fluorescence. Intrinsic and bis-ANS fluorescence analyses indicated that irradiation caused unfolding followed by aggregation of the lectin. Our results suggest that irradiation can lead to significant changes in the protein structure, which may promote the loss of its binding property and toxic action. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码：484 / 487
页数：4
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