Histamine production by the gut microbiota induces visceral hyperalgesia through histamine 4 receptor signaling in mice

被引：92

作者：

De Palma, Giada ^{[1
]}

Shimbori, Chiko ^{[1
]}

Reed, David E. ^{[2
]}

Yu, Yang ^{[2
]}

Rabbia, Virginia ^{[1
]}

Lu, Jun ^{[1
]}

Jimenez-Vargas, Nestor ^{[2
]}

Sessenwein, Jessica ^{[2
]}

Lopez-Lopez, Cintya ^{[2
]}

Pigrau, Marc ^{[1
]}

Jaramillo-Polanco, Josue ^{[2
]}

Zhang, Yong ^{[2
]}

Baerg, Lauren ^{[1
]}

Manzar, Ahmad ^{[1
]}

Pujo, Julien ^{[1
]}

Bai, Xiaopeng ^{[1
]}

Pinto-Sanchez, Maria Ines ^{[1
]}

Caminero, Alberto

Madsen, Karen ^{[3
]}

Surette, Michael G. ^{[1
]}

Beyak, Michael ^{[2
]}

Lomax, Alan E. ^{[2
]}

Verdu, Elena F. ^{[1
]}

Collins, Stephen M. ^{[1
]}

Vanner, Stephen J. ^{[2
]}

Bercik, Premysl ^{[1
]}

机构：

[1] McMaster Univ, Farncombe Family Digest Hlth Res Inst, Hamilton, ON, Canada

[2] Queens Univ, Gastrointestinal Dis Res Unit, Kingston, ON, Canada

[3] Univ Alberta, Edmonton, AB, Canada

来源：

SCIENCE TRANSLATIONAL MEDICINE | 2022年 / 14卷 / 655期

基金：

加拿大健康研究院;

关键词：

IRRITABLE-BOWEL-SYNDROME; CHAIN FATTY-ACIDS; H-4; RECEPTOR; ABDOMINAL-PAIN; MAST-CELLS; KEY; COMMUNITIES; SYMPTOMS; BACTERIA; MEDIATOR;

D O I：

10.1126/scitranslmed.abj1895

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The gut microbiota has been implicated in chronic pain disorders, including irritable bowel syndrome (IBS), yet specific pathophysiological mechanisms remain unclear. We showed that decreasing intake of fermentable carbohydrates improved abdominal pain in patients with IBS, and this was accompanied by changes in the gut microbiota and decreased urinary histamine concentrations. Here, we used germ-free mice colonized with fecal microbiota from patients with IBS to investigate the role of gut bacteria and the neuroactive mediator histamine in visceral hypersensitivity. Germ-free mice colonized with the fecal microbiota of patients with IBS who had high but not low urinary histamine developed visceral hyperalgesia and mast cell activation. When these mice were fed a diet with reduced fermentable carbohydrates, the animals showed a decrease in visceral hypersensitivity and mast cell accumulation in the colon. We observed that the fecal microbiota from patients with IBS with high but not low urinary histamine produced large amounts of histamine in vitro. We identified Klebsiella aerogenes, carrying a histidine decarboxylase gene variant, as a major producer of this histamine. This bacterial strain was highly abundant in the fecal microbiota of three independent cohorts of patients with IBS compared with healthy individuals. Pharmacological blockade of the histamine 4 receptor in vivo inhibited visceral hypersensitivity and decreased mast cell accumulation in the colon of germ-free mice colonized with the high histamine-producing IBS fecal microbiota. These results suggest that therapeutic strategies directed against bacterial histamine could help treat visceral hyperalgesia in a subset of patients with IBS with chronic abdominal pain.

引用

页数：12

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