Grafting of silica with a hydrophilic triol acrylamide polymer via surface-initiated "grafting from" method for hydrophilic-interaction chromatography

被引:26
作者
Peng, Xi-Tian [1 ]
Yuan, Bi-Feng [1 ]
Feng, Yu-Qi [1 ]
机构
[1] Wuhan Univ, Dept Chem, Minist Educ, Key Lab Analyt Chem Biol & Med, Wuhan 430072, Peoples R China
关键词
Grafting polymerization; Hydrophilic-interaction chromatography; Peptides; N-Acryloyltris(hydroxymethyl) aminomethane; Nucleosides; INTERACTION LIQUID-CHROMATOGRAPHY; CHIRAL STATIONARY-PHASE; MASS-SPECTROMETRY; CLICK CHEMISTRY; POLAR COMPOUNDS; SEPARATION; MODE; PERFORMANCE; PEPTIDES; HPLC;
D O I
10.1002/jssc.201100570
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A novel hydrophilic polymer-coated silica sorbent has been prepared using the radical grafting from polymerization method through surface-bound azo initiators for hydrophilic-interaction chromatography (HILIC). The azo groups were introduced to the surface of silica gel through the reaction with amino groups on the surface of silica gel with 4,4'-azobis(4-cyanopentanoic acid chloride) (ACVC). The resultant azo-immobilized silica gel served as surface initiator to polymerize hydrophilic triol acrylamide monomer N-acryloyltris(hydroxymethyl) aminomethane (NA) in methanol to get hydrophilic polymer-coated silica sorbent. The obtained poly(NA)-coated silica (pNA-sil) was characterized by Fourier transform infrared spectroscopy (FT-IR), elemental analysis (EA), and nitrogen sorption porosimetry (NSP). Then the pNA-sil was packed into the stainless-steel column and evaluated in high-performance liquid chromatography (HPLC). Good chromatographic performance for the separation of peptides and nucleosides was obtained under HILIC mode. The results indicated that the pNA-sil stationary phase behaved as mixed-mode retention mechanisms of hydrophilic and ionic interactions. Furthermore, the pNA-sil phase was used to separate tryptic digest of beta-casein and our results showed that more than 12 peptides peaks were resolved and well distributed within the elution window. Finally, the pNA-sil stationary phase was demonstrated to possess remarkable reproducibility and stability. Taken together, the pNA-sil stationary phase prepared in the current study offers a potential application in proteomics study.
引用
收藏
页码:3123 / 3130
页数:8
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