Studies on stability of Pd(II)ternary complexes and interaction with DNA

Two ternary complexes [Pd(bipy)(DL-gly)]Cl center dot 2H(2)O(1) and [Pd(bipy)(L-lys)]Cl center dot H2O(2) were synthesized. The stability constants 1g beta of two complexes are 13.81(1) and 13.71(2), respectively, and relative stability AlgK, IgX are higher than statistical value. The results show that there exists d-p pi electronic effect in complexes. Intercalation of the complexes with DNA can be demonstrated by LJV-Vis spectra and fluorescence spectra. The binding constans of the two complexes with sperm DNA are 2.37x10(6)(1) and 4.57x10(6)(2), respectively. It shows that the interaction intensity is 1 < 2.The CD spectra also show that the tow complexes were reacted with DNA. The results of gel electrophoresis experiments reveal that plasmid pBR322 DNA was cleaved by the two complexes when complex concentration ranges from 3.00x10(-3) to 7.50x10(-4) mol center dot L-1, whereas insertion is main binding model when complex concentration is lower than 3.75x 10(-1) mol center dot L-1.