Laminin-111 peptide C16 regulates invadopodia activity of malignant cells through β1 integrin, Src and ERK 1/2

被引：18

作者：

Siqueira, Adriane S. ^{[1
,6
]}

Pinto, Monique P. ^{[1
]}

Cruz, Mario C. ^{[2
]}

Smuczek, Basilio ^{[1
]}

Cruz, Karen S. P. ^{[3
]}

Barbuto, Jose Alexandre M. ^{[3
]}

Hoshino, Daisuke ^{[4
]}

Weaver, Alissa M. ^{[5
]}

Freitas, Vanessa M. ^{[1
]}

Jaeger, Ruy G. ^{[1
]}

机构：

[1] Univ Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, BR-05508000 Sao Paulo, SP, Brazil

[2] Univ Sao Paulo, Inst Biomed Sci, ICB Core Facil, BR-05508000 Sao Paulo, SP, Brazil

[3] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, BR-05508000 Sao Paulo, SP, Brazil

[4] Kanagawa Canc Ctr, Div Canc Cell Res, Yokohama, Kanagawa 2418515, Japan

[5] Vanderbilt Univ, Med Ctr, Dept Canc Biol, Nashville, TN 37232 USA

[6] Positivo Univ, Sch Dent, BR-81280330 Curitiba, PR, Brazil

来源：

ONCOTARGET | 2016年 / 7卷 / 30期

基金：

巴西圣保罗研究基金会; 美国国家卫生研究院;

关键词：

laminin; invadopodia; beta; 1; integrin; ERK; 1-2; pathway; Src kinases; CYSTIC CARCINOMA-CELL; PROTEASE ACTIVITY; MATRIX-METALLOPROTEINASE; CANCER; SITES; PODOSOMES; CORTACTIN; INVASION; IDENTIFICATION; METASTASIS;

D O I：

10.18632/oncotarget.10062

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Laminin peptides influence tumor behavior. In this study, we addressed whether laminin peptide C16 (KAFDITYVRLKF, gamma 1 chain) would increase invadopodia activity of cells from squamous cell carcinoma (CAL27) and fibrosarcoma (HT1080). We found that C16 stimulates invadopodia activity over time in both cell lines. Rhodamine-conjugated C16 decorates the edge of cells, suggesting a possible binding to membrane receptors. Flow cytometry showed that C16 increases activated beta 1 integrin, and beta 1 integrin miRNA-mediated depletion diminishes C16-induced invadopodia activity in both cell lines. C16 stimulates Src and ERK 1/2 phosphorylation, and ERK 1/2 inhibition decreases peptide-induced invadopodia activity. C16 also increases cortactin phosphorylation in both cells lines. Based on our findings, we propose that C16 regulates invadopodia activity over time of squamous carcinoma and fibrosarcoma cells, probably through beta 1 integrin, Src and ERK 1/2 signaling pathways.

引用

页码：47904 / 47917

页数：14

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