The Determination of Cannabinoids in Urine Samples Using Microextraction by Packed Sorbent and Gas Chromatography-Mass Spectrometry

被引：13

作者：

Rosendo, Luana M. ^{[1
]}

Rosado, Tiago ^{[1
,2
]}

Oliveira, Patrik ^{[1
]}

Simao, Ana Y. ^{[1
,2
]}

Margalho, Claudia ^{[3
]}

Costa, Suzel ^{[4
]}

Passarinha, Luis A. ^{[1
,2
,5
,6
]}

Barroso, Mario ^{[4
]}

Gallardo, Eugenia ^{[1
,2
]}

机构：

[1] Univ Beira Interior CICS UBI, Ctr Invest Ciencias Saude, Av Infante D Henrique, P-6201506 Covilha, Portugal

[2] Univ Beira Interior, Lab Farmacotoxicol, Ubimed, Estr Municipal 506, P-6200284 Covilha, Portugal

[3] Inst Med Legal & Ciencias Forenses, Delegacao Ctr, Serv Quim & Toxicol Forenses, P-3000213 Coimbra, Portugal

[4] Inst Med Legal & Ciencias Forenses Delegacao Sul, Serv Quim & Toxicol Forenses, P-1169201 Lisbon, Portugal

[5] Univ Nova Lisboa, Fac Ciencias & Tecnol, Dept Quim, UCIBIO Apllied Mol Bioesci Unit, P-1099085 Caparica, Portugal

[6] Univ NOVA, NOVA Sch Sci & Technol, Associate Lab I4HB, Inst Hlth & Bioecon, P-2819516 Caparica, Portugal

来源：

MOLECULES | 2022年 / 27卷 / 17期

关键词：

cannabinoids; Delta(9)-tetrahydrocannabinol; urine; microextraction by packed sorbent; gas chromatography-mass spectrometry; 11-NOR-DELTA(9)-TETRAHYDROCANNABINOL-9-CARBOXYLIC ACID; SENSITIVE DETERMINATION; MAIN METABOLITES; HUMAN PLASMA; LC-MS/MS; THC-COOH; LIQUID; QUANTIFICATION; DELTA(9)-TETRAHYDROCANNABINOL; CONFIRMATION;

D O I：

10.3390/molecules27175503

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cannabis is the most consumed illicit drug worldwide, and its legal status is a source of concern. This study proposes a rapid procedure for the simultaneous quantification of Delta(9)-tetrahydrocannabinol (THC), 11-hydroxy-Delta(9)-tetrahydrocannabinol (11-OH-THC), 11-nor-9-carboxy-Delta(9)-tetrahydrocannabinol (THC-COOH), cannabidiol (CBD), and cannabinol (CBN) in urine samples. Microextraction by packed sorbent (MEPS) was used to pre-concentrate the analytes, which were detected by gas chromatography-mass spectrometry. The procedure was previously optimized, and the final conditions were: conditioning with 50 mu L methanol and 50 mu L of water, sample load with two draw-eject cycles, and washing with 310 mu L of 0.1% formic acid in water with 5% isopropanol; the elution was made with 35 p.t of 0.1% ammonium hydroxide in methanol. This fast extraction procedure allowed quantification in the ranges of 1-400 ng/mL for THC and CBD, 5-400 ng/mL for CBN and 11-OH-THC, and 10-400 ng/mL for THC-COOH with coefficients of determination higher than 0.99. The limits of quantification and detection were between 1 and 10 ng/mL using 0.25 mL of sample. The extraction efficiencies varied between 26 and 85%. This analytical method is the first allowing the for determination of cannabinoids in urine samples using MEPS, a fast, simple, and low-cost alternative to conventional techniques.

引用

页数：21

共 65 条

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