Growth enhancement of shrimp (Litopenaeus schmitti) after transfer of tilapia growth hormone gene

被引：16

作者：

Arenal, Amilcar ^{[1
]}

Pimentel, Rafael ^{[1
]}

Pimentel, Eulogio ^{[1
]}

Martin, Leonardo ^{[1
]}

Santiesteban, Dayami ^{[1
]}

Franco, Ramon ^{[1
]}

Alestrom, Peter ^{[2
]}

机构：

[1] Ctr Genet Engn & Biotechnol, Camaguey 70100 1, Cuba

[2] Norwegian Sch Vet Sci, Dept Basic Sci & Aquat Med, N-0033 Oslo, Norway

来源：

BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 05期

关键词：

development index; growth hormone; penaeid; shrimp;

D O I：

10.1007/s10529-008-9636-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Electroporation of Litopenaeus schmitti embryos was used to transfer the pE300tiGH15 plasmid that contains the tilapia growth hormone gene (tiGH) complexed with a nuclear localization signal peptide into the zygotes. The gene construct was detected in 35 (36%) of the 98 larvae screened by PCR and Southern blot analyses. Western blot analyses revealed that 34% of the screened larvae expressed a single tiGH-specific band with the expected molecular mass (23.1 kDa). The development index and larval length indicated a significant growth enhancement from day 3 on after electroporation, with an average of 32% of the growth enhancement. To our knowledge, this is the first report on gene transfer enhanced growth in crustaceans.

引用

页码：845 / 851

页数：7

共 30 条

[1] Zebrafish in functional genomics and aquatic biomedicine [J].

Aleström, P ;

Holter, JL ;

Nourizadeh-Lillabadi, R .

TRENDS IN BIOTECHNOLOGY, 2006, 24 (01) :15-21

[2] The SV40 T antigen nuclear localization sequence enhances nuclear import of vector DNA in embryos of a crustacean (Litopenaeus schmitti) [J].

Arenal, A ;

Pimentel, R ;

García, C ;

Pimentel, E ;

Aleström, P .

GENE, 2004, 337 :71-77

[3]

Arenal Amilcar, 2000, Biotecnologia Aplicada, V17, P247

[4]

ARENAL F, 2002, C INT VIRT AC

[5] KNOWLEDGE AND RESEARCH PROSPECTS IN MARINE MOLLUSK AND CRUSTACEAN IMMUNOLOGY [J].

BACHERE, E ;

MIALHE, E ;

NOEL, D ;

BOULO, V ;

MORVAN, A ;

RODRIGUEZ, J .

AQUACULTURE, 1995, 132 (1-2) :17-32

[6]

Bensheng J, 1997, AQUAC RES, V28, P183

[7]

Cabrera E., 1995, Theriogenology, V43, P180, DOI 10.1016/0093-691X(95)92334-6

[8] Electroporation of DNA, RNA, and morpholinos into zebrafish embryos [J].

Cerda, Gustavo A. ;

Thomas, Jeanne E. ;

Allende, Miguel L. ;

Karlstrom, Rolf O. ;

Palma, Veronica .

METHODS, 2006, 39 (03) :207-211

[9]

CHARMANTIER G, 1989, CR ACAD SCI III-VIE, V308, P21

[10] The nuclear localization sequence of the SV40 Tantigen promotes transgene uptake and expression in zebrafish embryo nuclei [J].

Collas, P ;

Husebye, H ;

Alestrom, P .

TRANSGENIC RESEARCH, 1996, 5 (06) :451-458

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