MdMYB9 and MdMYB11 are Involved in the Regulation of the JA-Induced Biosynthesis of Anthocyanin and Proanthocyanidin in Apples

被引:303
作者
An, Xiu-Hong [1 ,2 ,3 ,4 ]
Tian, Yi [1 ,2 ,3 ,4 ]
Chen, Ke-Qin [1 ,2 ,3 ]
Liu, Xiao-Juan [1 ,2 ,3 ]
Liu, Dan-Dan [1 ,2 ,3 ]
Xie, Xing-Bin [1 ,2 ,3 ]
Cheng, Cun-Gang [4 ]
Cong, Pei-Hua [4 ]
Hao, Yu-Jin [1 ,2 ,3 ]
机构
[1] Shandong Agr Univ, Natl Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China
[2] Shandong Agr Univ, Natl Res Ctr Apple Engn & Technol, Tai An 271018, Shandong, Peoples R China
[3] Shandong Agr Univ, Coll Hort Sci & Engn, Tai An 271018, Shandong, Peoples R China
[4] CAAS, Res Inst Pomol, Xingcheng 125100, Peoples R China
基金
中国国家自然科学基金;
关键词
Anthocyanin; Apple; Jasmonate; JAZ; MYB transcription factors; Proanthocyanidin; MYB TRANSCRIPTION FACTOR; DOMAIN PROTEINS INTERACT; MOLECULAR ANALYSIS; INDUCED EXPRESSION; METHYL JASMONATE; FRUIT COLORATION; RED COLORATION; GENE ENCODES; ARABIDOPSIS; ACCUMULATION;
D O I
10.1093/pcp/pcu205
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Anthocyanin and proanthocyanidin (PA) are important secondary metabolites and beneficial to human health. Their biosynthesis is induced by jasmonate (JA) treatment and regulated by MYB transcription factors (TFs). However, which and how MYB TFs regulate this process is largely unknown in apple. In this study, MdMYB9 and MdMYB11 which were induced by methyl jasmonate (MeJA) were functionally characterized. Overexpression of MdMYB9 or MdMYB11 promoted not only anthocyanin but also PA accumulation in apple calluses, and the accumulation was further enhanced by MeJA. Subsequently, yeast two-hybrid, pull-down and bimolecular fluorescence complementation assays showed that both MYB proteins interact with MdbHLH3. Moreover, Jasmonate ZIM-domain (MdJAZ) proteins interact with MdbHLH3. Furthermore, chromatin immunoprecipitation-quantitative PCR and yeast one-hybrid assays demonstrated that both MdMYB9 and MdMYB11 bind to the promoters of ANS, ANR and LAR, whereas MdbHLH3 is recruited to the promoters of MdMYB9 and MdMYB11 and regulates their transcription. In addition, transient expression assays indicated that overexpression of MdJAZ2 inhibits the recruitment of MdbHLH3 to the promoters of MdMYB9 and MdMYB11. Our findings provide new insight into the mechanism of how MeJA regulates anthocyanin and PA accumulation in apple.
引用
收藏
页码:650 / 662
页数:13
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