Utilization of Corncob Biochar in Cultivation Media for Cordycepin Production and Biomass of Cordyceps militaris

被引:5
作者
Phoungthong, Khamphe [1 ,2 ]
Aiphuk, Waraporn [3 ]
Maneerat, Tharakorn [4 ,5 ,6 ]
Suwunwong, Thitipone [4 ,5 ]
Choto, Patcharanan [4 ,5 ]
Chomnunti, Putarak [3 ,4 ]
机构
[1] Prince Songkla Univ, Environm Assessment & Technol Hazardous Waste Man, Fac Environm Management, Hat Yai 90112, Thailand
[2] Ctr Excellence Hazardous Subst Management HSM, Bangkok 10330, Thailand
[3] Mae Fah Luang Univ, Ctr Excellence Fungal Res, Chiang Rai 57100, Thailand
[4] Mae Fah Luang Univ, Sch Sci, Chiang Rai 57100, Thailand
[5] Mae Fah Luang Univ, Ctr Chem Innovat Sustainabil, Chiang Rai 57100, Thailand
[6] Mae Fah Luang Univ, Med Plant Innovat Ctr, Chiang Rai 571006, Thailand
关键词
adenosine; agricultural residue; cultivation; cordycepin; SOLID-STATE FERMENTATION; CULTURE-CONDITIONS; FRUITING BODY; ENHANCED PRODUCTION; SUBMERGED CULTURE; OPTIMIZATION; GROWTH; INGREDIENTS; FUNGI;
D O I
10.3390/su14159362
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Cordyceps militaris is an entomopathogenic fungus. It is well-known as a rich source of bioactive compounds called cordycepins and adenosines, which are useful in medicinal applications. The effects of medium components on cordycepin and adenosine production by C. militaris, obtained by adding different conditions of corncob biochar in solid media, were investigated in this study. The medium components, which mixed 0.1, 0.3, 0.5, 1, 5 and 10 g of biochar with rice berries, were optimized to improve the yield of biomasses, cordycepins, and adenosines. The results showed that 10 g of biochar mixed with a rice berry medium was the optimal medium condition for the highest dry fruiting body weight (DFW) and cordycepin yield (CY) at 3.6 kg/bottle and 20.5 mg/g, respectively, but the adenosine yield (AY) was similar to that in other conditions. Moreover, the SEM showed that the mycelia of C. militaris attached to the biochar surface (pores) and used it as the resident. EDS analysis from the basal medium indicated that C and O were present in the mycelia of C. militaris with the average values of 25.6% and 71.4%, respectively. This study provides an effective cultivation method by using agricultural residue, and biochar corncob as a high concentration of carbon for increasing the biomass, cordycepin, and adenosine yield of C. militaris. The information obtained in this study is fundamental and useful to the development of a C. militaris cultivation process for the efficient production of cordycepin on a large scale. The findings suggest that the system design of the cultivation medium is crucial for growth and cordycepin production.
引用
收藏
页数:10
相关论文
共 50 条
  • [31] Enhanced production of cordycepin under solid-state fermentation of Cordyceps militaris by using combinations of grains/substrates
    Mahesh Borde
    Sanjay K. Singh
    Brazilian Journal of Microbiology, 2023, 54 : 2765 - 2772
  • [32] Enhanced production of cordycepin by surface culture using the medicinal mushroom Cordyceps militaris
    Masuda, Mina
    Urabe, Eriko
    Honda, Hiromitsu
    Sakurai, Akihiko
    Sakakibara, Mikio
    ENZYME AND MICROBIAL TECHNOLOGY, 2007, 40 (05) : 1199 - 1205
  • [33] Efficient production of cordycepin by the Cordyceps militaris mutant G81-3 for practical use
    Masuda, Mina
    Das, Shonkor Kumar
    Hatashita, Masanori
    Fujihara, Shinya
    Sakurai, Akihiko
    PROCESS BIOCHEMISTRY, 2014, 49 (02) : 181 - 187
  • [34] Biomass and Cordycepin Production by the Medicinal Mushroom Cordyceps militaris-A Review of Various Aspects and Recent Trends towards the Exploitation of a Valuable Fungus
    Kontogiannatos, Dimitrios
    Koutrotsios, Georgios
    Xekalaki, Savvina
    Zervakis, Georgios I.
    JOURNAL OF FUNGI, 2021, 7 (11)
  • [35] Two-step Shake-static Fermentation to Enhance Cordycepin Production by Cordyceps militaris
    Tang, Jiapeng
    Qian, Zhenqing
    Zhu, Li
    IAEDS15: INTERNATIONAL CONFERENCE IN APPLIED ENGINEERING AND MANAGEMENT, 2015, 46 : 19 - 24
  • [36] Effect of the salts of deep ocean water on the production of cordycepin and adenosine of Cordyceps militaris-fermented product
    Yu-Ping Hung
    Jyh-Jye Wang
    Bai-Luh Wei
    Chun-Lin Lee
    AMB Express, 5
  • [37] Adenosine Deaminase Inhibitory Activity of Medicinal Plants: Boost the Production of Cordycepin in Cordyceps militaris
    Turk, Ayman
    Lee, Solip
    Yeon, Sang Won
    Ryu, Se Hwan
    Han, Yoo Kyong
    Kim, Young Jun
    Ko, Sung Min
    Kim, Beom Seok
    Hwang, Bang Yeon
    Lee, Ki Yong
    Lee, Mi Kyeong
    ANTIOXIDANTS, 2023, 12 (06)
  • [38] Effect of light-emitting diodes on the production of cordycepin, mannitol and adenosine in solid-state fermented rice by Cordyceps militaris
    Chiang, Shen-Shih
    Liang, Zeng-Chin
    Wang, Yu-Chi
    Liang, Chih-Hung
    JOURNAL OF FOOD COMPOSITION AND ANALYSIS, 2017, 60 : 51 - 56
  • [39] Effects of additives on cordycepin production using a Cordyceps militaris mutant induced by ion beam irradiation
    Das, S. K.
    Masuda, M.
    Sakurai, A.
    Sakakibara, M.
    AFRICAN JOURNAL OF BIOTECHNOLOGY, 2009, 8 (13): : 3041 - 3047
  • [40] Effect of pH, temperature and incubation time on cordycepin production from Cordyceps militaris using solid-state fermentation on various substrates
    Adnan, Mohd
    Ashraf, Syed Amir
    Khan, Saif
    Alshammari, Eyad
    Awadelkareem, Amir Mahgoub
    CYTA-JOURNAL OF FOOD, 2017, 15 (04) : 617 - 621