Tools to evaluate the conformation of protein products

被引：19

作者：

Manta, Bruno ^{[2
,3
]}

Obal, Gonzalo ^{[1
]}

Ricciardi, Alejandro ^{[4
]}

Pritsch, Otto ^{[1
,5
]}

Denicola, Ana ^{[2
]}

机构：

[1] Inst Pasteur Montevideo, Unidad Biofis Prot, Montevideo 11400, Uruguay

[2] Univ Republica, Lab Fisicoquim Biol, Inst Quim Biol, Fac Ciencias, Montevideo, Uruguay

[3] Inst Pasteur Montevideo, Grp Biol Redox Trypanosomas, Montevideo 11400, Uruguay

[4] Inst Pasteur Montevideo, Lab Control Biofarmacos, Montevideo 11400, Uruguay

[5] Univ Republica, Dept Inmunobiol, Fac Med, Montevideo, Uruguay

来源：

BIOTECHNOLOGY JOURNAL | 2011年 / 6卷 / 06期

关键词：

Circular dichroism; Isothermal titration calorimetry; Fluorescence; Protein folding; Surface plasmon resonance; SURFACE-PLASMON RESONANCE; ISOTHERMAL TITRATION CALORIMETRY; ANALYTICAL ULTRACENTRIFUGATION; LIGHT-SCATTERING; INFRARED-SPECTROSCOPY; BIOLOGICAL ASSAYS; ESCHERICHIA-COLI; MEMBRANE-PROTEIN; CYTOCHROME-C; SIDES;

D O I：

10.1002/biot.201100107

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Production of recombinant proteins is a process intensively used in the research laboratory. In addition, the main biotechnology market products are recombinant proteins and monoclonal antibodies. The biological (and clinical) properties of the protein product strongly depend on the conformation of the polypeptide. Therefore, assessment of the correct conformation of the produced protein is crucial. There is no single method to assess every aspect of protein structure or function. Depending on the protein, the methods of choice vary. There are general methods to evaluate not only mass and primary sequence of the protein, but also higher-order structure. This review outlines the principal techniques for determining the conformation of a protein from structural (biophysical methods) to functional (in vitro binding assays) analyses.

引用

页码：731 / 741

页数：11

共 77 条

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