Characterization of Human Bladder Cell Membrane During Cancer Transformation

被引：33

作者：

Dobrzynska, Izabela ^{[1
]}

Szachowicz-Petelska, Barbara ^{[1
]}

Darewicz, Barbara ^{[2
]}

Figaszewski, Zbigniew A. ^{[1
,3
]}

机构：

[1] Univ Bialystok, Inst Chem, PL-15443 Bialystok, Poland

[2] Med Univ Biaystok, Clin Dept Urol, PL-15276 Biaystok, Poland

[3] Univ Warsaw, Lab Electrochem Power Sources, Fac Chem, PL-02093 Warsaw, Poland

来源：

JOURNAL OF MEMBRANE BIOLOGY | 2015年 / 248卷 / 02期

关键词：

Phospholipids; Proteins; Electric charge; Human bladder; ELECTRIC CHARGE; PURIFICATION;

D O I：

10.1007/s00232-015-9770-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phenomena associated with changes in cell membranes are thought to play an important role in the cancer transformation. We hypothesized that the electrical charge of tumor cells can indirectly represent membrane-based changes that have occurred during cell transformation and may indicate tumor cell status. Here, we describe work showing that phospholipids, proteins content, and electric charge, are all altered in the cell membranes of pT2 stage/grade G3 bladder cancer. Qualitative and quantitative phospholipid composition and the presence of integral membrane proteins were identified using high-performance liquid chromatography. Protein composition was determined using selective hydrolysis of isolated bladder cell membrane proteins and peptide resolution. The surface charge density of human bladder cell membranes was determined using electrophoresis. Our results show that cancer transformation is associated with increased phospholipid levels and a decreased level of integral proteins. Moreover, the process of cancer transformation significantly enhanced changes in the surface charge density of the human bladder cell membrane. In conclusion, this study demonstrates that cell membrane structure and function are modified in bladder cancer cells and that further work in this area is warranted.

引用

页码：301 / 307

页数：7

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