Inhibition of DLC-1 gene expression by RNA interference in the colon cancer LoVo cell line

被引：6

作者：

Jin, Yueling ^{[1
]}

Tian, Xiaoqiang ^{[1
]}

Shang, Yanfang ^{[1
]}

Huang, Peiling ^{[1
]}

机构：

[1] Southeast Univ, Sch Clin Med, Dept Clin Med, Nanjing 210009, Peoples R China

来源：

ONCOLOGY REPORTS | 2008年 / 19卷 / 03期

关键词：

RNA interference; deleted in liver cancer-1 gene; proliferation; cell cycle; cell migration;

D O I：

暂无

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

DLC-1 (deleted in liver cancer-1) is a potential tumor suppressor gene, which is inactive in liver carcinogenesis. To observe the effects of DLC-1 gene expression on cell proliferation and migration in the human colon cancer cell line, RNAi Lipo-recombinant of the DLC-1 gene (pGCsil-DLC-1) was constructed and transduced into LoVo cells which are positive for DLC-1 gene expression. Results showed that the RNAi recombinant effectively inhibited the expression of the DLC-1 gene in LoVo cells. Additionally, our data showed decreased DLC-1 gene expression which resulted in the promotion of LoVo cell proliferation. Flow cytometry in cell cycle detection further indicated that the DLC-1 gene induced cell cycle arrest at G2/M and a cell migration assay confirmed that the knocking down of DLC-1 gene expression promotes LoVo cell migration. Our observations suggest that the DLC-1 gene is associated with LoVo cell proliferation, migration and cell cycle distribution. DLC-1 is a potential suppressor gene in the colon cancer LoVo cell line and may play an important role in colon cancer mechanisms.

引用

页码：669 / 674

页数：6

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