A new thermostable β-glucosidase mined from Dictyoglomus thermophilum: Properties and performance in octyl glucoside synthesis at high temperatures

被引：28

作者：

Zou, Zheng-Zheng ^{[1
]}

Yu, Hui-Lei ^{[1
]}

Li, Chun-Xiu ^{[1
]}

Zhou, Xin-Wen ^{[2
,3
]}

Hayashi, Chihiro ^{[4
]}

Sun, Jie ^{[1
]}

Liu, Bao-Hong ^{[2
,3
]}

Imanaka, Tadeyuki ^{[1
,4
]}

Xu, Jian-He ^{[1
]}

机构：

[1] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biocatalysis & Synthet Biotechnol, Shanghai 200237, Peoples R China

[2] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China

[3] Fudan Univ, Inst Biomed Sci, Shanghai 200433, Peoples R China

[4] Ritsumeikan Univ, Coll Life Sci, Dept Biotechnol, Shiga 5258577, Japan

来源：

BIORESOURCE TECHNOLOGY | 2012年 / 118卷

基金：

中国国家自然科学基金;

关键词：

beta-Glucosidase; Dictyoglomus thermophilum; Thermostability; Octyl glucoside; Reverse hydrolysis; D-GLUCOPYRANOSIDES; ENZYMATIC CATALYSIS; PYROCOCCUS-FURIOSUS; REVERSE HYDROLYSIS; BIPHASIC SYSTEM; APPLE SEED; GENE; PURIFICATION; EXPRESSION; ALCOHOLS;

D O I：

10.1016/j.biortech.2012.04.040

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

A new beta-glucosidase (DtGH) representing 40% identity with an apple seed glycosidase (ASG) was cloned from Dictyoglomus thermophilum. DtGH showed extremely high thermostability in aqueous solution, with half-lives of 533, 44, and 5 h measured at 70, 80 and 90 degrees C, respectively. Therefore it was used for direct glycosylation of n-octanol at 70 degrees C instead of 50 degrees C as usually. As a result, the glucose based conversion was increased by 27%, but the time spent to reach equilibrium was decreased from 7 d to 3 d. This enzyme also exhibited excellent stability under the reaction environment, retaining 70-80% of its initial activity after 7 d of incubation at 70 degrees C in either 1.7 M glucose solution or octanol-aqueous (85:15, v/v) system. It could retain part of synthetic activity even in boiling water. Owing to the strong glucose-tolerance and extremely high thermostability, DtGH should be promising for various glucosides synthesis. Crown Copyright (c) 2012 Published by Elsevier Ltd. All rights reserved.

引用

页码：425 / 430

页数：6

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