Characterization and quantification of grape variety by means of shikimic acid concentration and protein fingerprint in still white wines

被引:25
作者
Chabreyrie, David [1 ]
Chauvet, Serge [1 ]
Guyon, Francois [1 ]
Salagoity, Marie-Helene [1 ]
Antinelli, Jean-Francois [2 ]
Medina, Bernard [1 ]
机构
[1] Serv Commun Lab, Lab Pessac, F-33408 Pessac, France
[2] Analyt Consulting, F-06000 Nice, France
关键词
proteins; dialyze; shikimic acid; high-performance capillary electrophoresis; grape varietal recognition; still white wine; chemometric; partial least-squares (PLS);
D O I
10.1021/jf800117k
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Protein profiles, obtained by high-performance capillary electrophoresis (HPCE) on white wines previously dialyzed, combined with shikimic acid concentration and multivariate analysis, were used for the determination of grape variety composition of a still white wine. Six varieties were studied through monovarietal wines elaborated in the laboratory: Chardonnay (24 samples), Chenin (24), Petit Manseng (7), Sauvignon (37), Semillon (24), and Ugni Blanc (9). Homemade mixtures were elaborated from authentic monovarietal wines according to a Plackett-Burman sampling plan. After protein peak area normalization, a matrix was elaborated containing protein results of wines (mixtures and monovarietal). Partial least-squares processing was applied to this matrix allowing the elaboration of a model that provided a varietal quantification precision of around 20% for most of the grape varieties studied. The model was applied to commercial samples from various geographical origins, providing encouraging results for control purposes.
引用
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页码:6785 / 6790
页数:6
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