Simple and specific detection of Bordetella holmesii by using a loop-mediated isothermal amplification assay

被引：10
作者：
Otsuka, Nao [1 ]
Yoshino, Shuji [2 ]
Kawano, Kimiko [2 ]
Toyoizumi-Ajisaka, Hiromi [1 ]
Shibayama, Keigo [1 ]
Kamachi, Kazunari [1 ]
机构：
[1] Natl Inst Infect Dis, Dept Bacteriol 2, Musashimurayama, Tokyo 2080011, Japan
[2] Miyazaki Prefectural Inst Publ Hlth & Environm, Miyazaki 8892155, Japan
来源：
MICROBIOLOGY AND IMMUNOLOGY | 2012年 / 56卷 / 07期
关键词：
Bordetella holmesii; loop-mediated isothermal amplification; molecular diagnosis; recA; PERTUSSIS-LIKE SYMPTOMS; SUSPECTED PERTUSSIS; PCR; BACTEREMIA; SEPTICEMIA; INFECTION; DIAGNOSIS; ANEMIA; DNA;
D O I：
10.1111/j.1348-0421.2012.00465.x
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
A loop-mediated isothermal amplification (LAMP) assay for simple detection of Bordetella holmesii was developed. This assay discriminates between B. holmesii and other Bordetella species and successfully detect B. holmesii DNA in nasopharyngeal swab samples from subjects with suspected pertussis. The LAMP assay results were in complete agreement with the results of previously published real-time PCR assay, indicating that the former is a powerful tool for the accurate diagnosis and surveillance of B. holmesii.
引用
收藏
页码：486 / 489
页数：4
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