E-cadherin mediates adhesion and endocytosis of Aspergillus fumigatus blastospores in human epithelial cells

被引：16

作者：

Xu Xiao-yong ^{[1
]}

Shi Yi ^{[1
]}

Zhang Peng-peng ^{[1
]}

Zhang Feng ^{[1
]}

Shen Yu-ying ^{[1
]}

Su Xin ^{[1
]}

Zhao Bei-lei ^{[1
]}

机构：

[1] Second Mil Med Univ, Dept Resp Med, Nanjing Gen Hosp, Nanjing Mil Command, Nanjing 210002, Jiangsu, Peoples R China

来源：

CHINESE MEDICAL JOURNAL | 2012年 / 125卷 / 04期

基金：

中国国家自然科学基金;

关键词：

E-cadherin; receptor; A549; endocytosis; Aspergillus fumigatus; PROTEIN; RECEPTORS; INVASION;

D O I：

10.3760/cma.j.issn.0366-6999.2012.04.011

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background Aspergillus fumigatus (A. fumigatus) is a ubiquitous saprophytic fungus responsible for the majority of invasive mold infections in patients undergoing chemotherapy, organ transplantation or with persistent neutropenia. This study aimed to determine the role of E-cadherin for adhesion and endocytosis of A. fumigatus blastospores in the human epithelial cell line A549. Methods A. fumigatus blastospores were incubated with the total protein of A549 to investigate the binding of E-cadherin and blastospores followed by an affinity purification procedure. After establishing the adhesion model, the adhesion and endocytosis of A. fumigatus blastospores by A549 cells were evaluated by down-regulating E-cadherin of A549 cells using blocking antibody or small interfering RNA (siRNA). Results E-cadherin was adhered to the surface of A. fumigatus blastospore. Adhesion and endocytosis of the blastospores were reduced by blocking or down-regulating E-cadherin in A549 cells. Conclusions E-cadherin is a receptor for adhesion and endocytosis of A. fumigatus blastospores in epithelial cells. This may open a new approach to treat this fungal infection. Chin Med J 2012;125(4):617-621

引用

页码：617 / 621

页数：5

共 13 条

[1] E-cadherin is a receptor for the common protein pneumococcal surface adhesin A (PsaA) of Streptococcus pneumoniae [J].