Phenotypical Analysis of Lymphocytes with Suppressive and Regulatory Properties (Tregs) and NK Cells in the Papillary Carcinoma of Thyroid

被引：86

作者：

Gogali, Foteini ^{[1
]}

Paterakis, George ^{[4
]}

Rassidakis, George Z. ^{[3
]}

Kaltsas, Gregory ^{[2
]}

Liakou, Chrysoula I. ^{[1
]}

Gousis, Panagiotis ^{[5
]}

Neonakis, Evangelos ^{[6
]}

Manoussakis, Menelaos N. ^{[2
]}

Liapi, Charis ^{[1
]}

机构：

[1] Univ Athens, Sch Med, Dept Pharmacol, Athens 11527, Greece

[2] Univ Athens, Sch Med, Dept Pathophysiol, Athens 11527, Greece

[3] Univ Athens, Sch Med, Dept Pathol 1, Athens 11527, Greece

[4] Athens Reg Gen Hosp G Gennimatas, Dept Immunol, Flow Cytometry Lab, Athens, Greece

[5] Metropolitan Hosp, Dept Endocrine Surg, Athens 18547, Greece

[6] Naval Hosp, Dept Surg, Athens 11521, Greece

来源：

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM | 2012年 / 97卷 / 05期

关键词：

NATURAL-KILLER-CELLS; MEMORY T-CELLS; IMMUNE DYSREGULATION; PERIPHERAL-BLOOD; FOXP3; EXPRESSION; IL-7; RECEPTOR; CANCER; POLYENDOCRINOPATHY; ENTEROPATHY; POPULATION;

D O I：

10.1210/jc.2011-1838

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Context: The immune system seems to play a key role in preventing metastasis and recurrence of thyroid cancer. T regulatory lymphocytes (Tregs) and natural killer (NK) cells play an important role in the dysfunction of the host immune system in cancer patients. Objective: We investigated thyroid gland infiltration by Tregs and NK cells in patients with papillary thyroid cancer (PTC) and thyroid nodular goiter (TNG). The correlation between the extent of the disease and the lymphocytic infiltration of Tregs and NK cells was examined. Design, Setting, and Participants: A total of 65 patients with PTC, 25 with TNG, and 50 healthy controls were studied. Blood and tissue samples from 28 patients with PTC and 13 with TNG and blood samples from the healthy controls were analyzed for T4 (CD3(+)CD4(+)), T8 (CD3(+)CD8(+)), NK (CD3(-)CD16(+)CD56(+)), and CD4(+)CD25(+)CD127(-/low) Tregs by flow cytometry (FC). Tissue samples were also analyzed for Foxp3(+) Tregs by immunohistochemistry. Results: Tregs showed greater infiltration in thyroid tissue of PTC patients compared with patients with TNG (P < 0.0009 for FC and P < 0.0001 for immunohistochemistry); FC analysis of blood samples showed no difference between the groups. Flow cytometry analysis showed significantly increased NK cells in PTC tissue compared with TNG tissue (P = 0.037), whereas blood samples showed no difference. CD4(+) and CD8(+) T cells did not differ in blood and tissue samples. Increased Tregs tissue infiltration was positively correlated with advanced disease stage (P < 0.0026), whereas NK infiltration was negatively correlated (P < 0.0041). Conclusion: Tregs and NK cells may be important regulators of thyroid cancer progression. (J Clin Endocrinol Metab 97: 1474-1482, 2012)

引用

页码：1474 / 1482

页数：9

共 44 条

[11] Increasing incidence of thyroid cancer in the United States, 1973-2002 [J].

Davies, L ;

Welch, HG .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2006, 295 (18) :2164-2167

[12] CD4+ Tregs and immune control [J].

Fehérvari, Z ;

Sakaguchi, S .

JOURNAL OF CLINICAL INVESTIGATION, 2004, 114 (09) :1209-1217

[13] Foxp3 Programs the Development and Function of CD4+CD25+ Regulatory T Cells (Reprinted from vol 4, pg 330-336, 2003) [J].

Fontenot, Jason D. ;

Gavin, Marc A. ;

Rudensky, Alexander Y. .

JOURNAL OF IMMUNOLOGY, 2017, 198 (03) :986-992

[14] Tissue digestion with dispase substantially reduces lymphocyte and macrophage cell-surface antigen expression [J].

Ford, AL ;

Foulcher, E ;

Goodsall, AL ;

Sedgwick, JD .

JOURNAL OF IMMUNOLOGICAL METHODS, 1996, 194 (01) :71-75

[15] Phase II clinical studies of denileukin diftitox diphtheria toxin fusion protein in patients with previously treated chronic lymphocytic leukemia [J].

Frankel, AE ;

Surendranathan, A ;

Black, JH ;

White, A ;

Ganjoo, K ;

Cripe, LD .

CANCER, 2006, 106 (10) :2158-2164

[16] Tumor-Associated Lymphocytes and Increased FoxP3+ Regulatory T Cell Frequency Correlate with More Aggressive Papillary Thyroid Cancer [J].

French, Jena D. ;

Weber, Zachary J. ;

Fretwell, Deborah L. ;

Said, Sherif ;

Klopper, Joshua P. ;

Haugen, Bryan R. .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2010, 95 (05) :2325-2333

[17] Cutting edge:: Emergence of CD127high functionally competent memory T cells is compromised by high viral loads and inadequate T cell help [J].

Fuller, MJ ;

Hildeman, DA ;

Sabbaj, S ;

Gaddis, DE ;

Tebo, AE ;

Shang, L ;

Goepfert, PA ;

Zajac, AJ .

JOURNAL OF IMMUNOLOGY, 2005, 174 (10) :5926-5930

[18] Immune dysregulation, polyendocrinopathy, enteropathy, and X-linked inheritance (IPEX), a syndrome of systemic autoimmunity caused by mutations of FOXP3, a critical regulator of T-cell homeostasis [J].

Gambineri, E ;

Torgerson, TR ;

Ochs, HD .

CURRENT OPINION IN RHEUMATOLOGY, 2003, 15 (04) :430-435

[19] FOXP3+ Tregs and B7-HI+/PD-I+T lymphocytes co-infiltrate the tumor tissues of high-risk breast cancer patients:: Implication for immunotherapy [J].

Ghebeh, Hazem ;

Barhoush, Eman ;

Tulbah, Asma ;

Elkum, Naser ;

Al-Tweigeri, Taher ;

Dermime, Said .

BMC CANCER, 2008, 8 (1)

[20] The role of regulatory T cells in the control of natural killer cells: relevance during tumor progression [J].

Ghiringhelli, Francois ;

Menard, Cedric ;

Martin, Francois ;

Zitvogel, Laurence .

IMMUNOLOGICAL REVIEWS, 2006, 214 :229-238

← 1 2 3 4 5 →