Detection of mycobacterial antibodies in serum samples by enzyme linked immunosorbent assay

被引：0

作者：

Nath, Sooraj S. ^{[1
]}

Nathiya, K. ^{[1
]}

Dhanabalan, R. ^{[2
]}

Angayarkanni, J. ^{[3
,4
]}

Palaniswamy, M. ^{[1
]}

机构：

[1] Karpagam Univ, Dept Microbiol, Coimbatore 641021, Tamil Nadu, India

[2] RVS Coll Arts & Sci, Dept Microbiol, Coimbatore 641402, Tamil Nadu, India

[3] Bharathiar Univ, Dept Microbial Biotechnol, Coimbatore 641046, Tamil Nadu, India

[4] Natl Univ Singapore, Dept Biol, Singapore 117548, Singapore

来源：

AFRICAN JOURNAL OF BIOTECHNOLOGY | 2011年 / 10卷 / 71期

关键词：

Enzyme-linked immunosorbent assay (ELISA); mycobacterial antigens; tuberculosis; TUBERCULOUS MENINGITIS; CEREBROSPINAL-FLUID; DIAGNOSIS; ANTIGENS; IGG;

D O I：

10.5897/AJB11.1302

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Tuberculosis (TB) is a common and deadly infectious disease caused by Mycobacterium tuberculosis and rarely by other subspecies of the M. tuberculosis complex, such as Mycobacterium bovis. Enzyme-linked immunosorbent assay (ELISA) for diagnosis of tuberculosis has been widely explored over the years. The purpose of this study was to reduce the time duration required for the diagnosis of tuberculosis. We have extracted antigens from the M. tuberculosis strain H37Ra. Antibody response was studied using ELISA. A total of 30 samples were analysed for their specificity and sensitivity. Of which, 20 were positive and the sensitivity and specificity of the test appeared to be 89%. The high sensitivities, specificities and promising antigenic combination in detection of TB suggest their potential application in diagnosis of TB.

引用

页码：16012 / 16015

页数：4

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