Integrated analysis of phenome, genome, and transcriptome of hybrid rice uncovered multiple heterosis-related loci for yield increase

被引：134

作者：

Li, Dayong ^{[1
]}

Huang, Zhiyuan ^{[2
]}

Song, Shuhui ^{[3
]}

Xin, Yeyun ^{[2
]}

Mao, Donghai ^{[4
]}

Lv, Qiming ^{[1
,2
]}

Zhou, Ming ^{[1
]}

Tian, Dongmei ^{[3
]}

Tang, Mingfeng ^{[4
]}

Wu, Qi ^{[1
]}

Liu, Xue ^{[5
]}

Chen, Tingting ^{[3
]}

Song, Xianwei ^{[1
]}

Fu, Xiqin ^{[2
]}

Zhao, Bingran ^{[2
]}

Liang, Chengzhi ^{[1
]}

Li, Aihong ^{[6
]}

Liu, Guozhen ^{[7
]}

Li, Shigui ^{[8
]}

Hu, Songnian ^{[5
]}

Cao, Xiaofeng ^{[1
]}

Yu, Jun

Yuan, Longping ^{[2
]}

Chen, Caiyan ^{[4
]}

Zhu, Lihuang ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Inst Genet & Dev Biol, Beijing 100101, Peoples R China

[2] Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha 410125, Hunan, Peoples R China

[3] Chinese Acad Sci, Beijing Inst Genom, BIG Data Ctr, Beijing 100101, Peoples R China

[4] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Hunan, Peoples R China

[5] Chinese Acad Sci, Beijing Inst Genom, Key Lab Genome Sci & Informat, Beijing 100101, Peoples R China

[6] Lixiahe Agr Res Inst Jiangsu Prov, Yangzhou 225007, Jiangsu, Peoples R China

[7] Hebei Agr Univ, Coll Life Sci, Baoding 071001, Peoples R China

[8] Sichuan Agr Univ, Rice Res Inst, Chengdu 611130, Peoples R China

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2016年 / 113卷 / 41期

基金：

中国国家自然科学基金;

关键词：

hybrid rice; heterosis; yield; QTL; RH8; ARABIDOPSIS HYBRIDS; GENETIC-BASIS; GRAIN-SIZE; INBREEDING DEPRESSION; ECTOPIC EXPRESSION; HEADING DATE; ORYZA-SATIVA; DOMINANCE; TRAITS; ALLELE;

D O I：

10.1073/pnas.1610115113

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Hybrid rice is the dominant form of rice planted in China, and its use has extended worldwide since the 1970s. It offers great yield advantages and has contributed greatly to the world's food security. However, the molecular mechanisms underlying heterosis have remained a mystery. In this study we integrated genetics and omics analyses to determine the candidate genes for yield heterosis in a model two-line rice hybrid system, Liang-you-pei 9 (LYP9) and its parents. Phenomics study revealed that the better parent heterosis (BPH) of yield in hybrid is not ascribed to BPH of all the yield components but is specific to the BPH of spikelet number per panicle (SPP) and paternal parent heterosis (PPH) of effective panicle number (EPN). Genetic analyses then identified multiple quantitative trait loci (QTLs) for these two components. Moreover, a number of differentially expressed genes and alleles in the hybrid were mapped by transcriptome profiling to the QTL regions as possible candidate genes. In parallel, a major QTL for yield heterosis, rice heterosis 8 (RH8), was found to be the DTH8/Ghd8/LHD1 gene. Based on the shared allelic heterozygosity of RH8 in many hybrid rice cultivars, a common mechanism for yield heterosis in the present commercial hybrid rice is proposed.

引用

页码：E6026 / E6035

页数：10

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