Simultaneous Detection and Virulence Characterisation of Dichelobacter nodosus from Ovine Footrot by Multiplex PCR

被引：0

作者：

Taku, A. K. ^{[1
]}

Beigh, M. M. ^{[1
]}

Kumar, B. ^{[1
]}

Kumar, S. ^{[1
]}

Bhat, M. A. ^{[1
]}

机构：

[1] Sher E Kashmir Univ Agr Sci & Technol Jammu, Div Vet Microbiol & Immunol, Jammu 181102, India

来源：

JOURNAL OF ANIMAL AND VETERINARY ADVANCES | 2011年 / 10卷 / 24期

关键词：

Footrot; Gelatin gel test; intA gene; multiplex PCR; 16S rRNA; India; POLYMERASE-CHAIN-REACTION; BACTEROIDES-NODOSUS; EXTRACELLULAR PROTEASES; KASHMIR; BENIGN; INDIA; SHEEP; PATHOGENESIS; ROT;

D O I：

暂无

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Out of 262 swab samples from feet of clinically footrot affected sheep from Jammu and Kashmir, 135 (52.0%) detected positive for Dichelobacter nodosus by 16S rRNA gene specific PCR. Out of these 135 positive samples, 82 (61.74%) tested positive for intA gene carried by virulent strains. A mulplex PCR for simultaneous detection and virulence characterization of D. nodosus in clinical samples was therefore devised. The test detected 77 (57.03%) samples positive for virulent D. nodosus. The results were comparable and the success of the multiplex PCR was established. Out of 30 randomly selected isolates subjected to gelatin gel test, 24 isolates with intA gene produced thermostable protease while six isolates without intA gene revealed the production of thermolabile protease. This indicated a good co-relation between presence of intA gene and gelatine gel test in determination of the D. nodosus virulence.

引用

页码：3192 / 3195

页数：4

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