Structure elucidation and gene cluster annotation of the O-antigen of Vibrio cholerae O100 containing two rarely occurred amino sugar derivatives

被引：5
作者：
Perepelov, Andrei, V [1 ]
Guo, Xi [2 ]
Filatov, Andrei, V [1 ]
Shashkov, Alexander S. [1 ]
Senchenkova, Sofia N. [1 ]
Li, Bin [2 ]
机构：
[1] Russian Acad Sci, ND Zelinsky Inst Organ Chem, Moscow, Russia
[2] Nankai Univ, TEDA, TEDA Inst Biol Sci & Biotechnol, Tianjin, Peoples R China
来源：
CARBOHYDRATE RESEARCH | 2019年 / 472卷
基金：
俄罗斯科学基金会;
关键词：
Vibrio cholerae; Bacterial polysaccharide structure; O-antigen; O-specific polysaccharide; Lipopolysaccharide; O-antigen gene cluster; SOMATIC ANTIGENS; POLYSACCHARIDE; LIPOPOLYSACCHARIDES; PSEUDOMONADS; CHAINS;
D O I：
10.1016/j.carres.2018.11.001
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
O-polysaccharide (O-antigen) was isolated from the lipopolysaccharide of Vibrio cholerae O100 and studied by component analyses and 1D and 2D NMR spectroscopy. The following structure of the O-polysaccharide was established: -> 3)-beta-D-QuipNAc4N(dHh)-(1 -> 3)-alpha-D-Fucp4N(RHb)-(1 -> 3)-alpha-L-FucpNAc-(1 -> where Hb and dHh indicate 3-hydroxybutanoyl and 3,5-dihydroxyhexanoyl, respectively. The O-antigen gene cluster of V. cholerae O100 has been sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in agreement with the OPS structure.
引用
收藏
页码：98 / 102
页数：5
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