Epiphytic fitness of Pseudomonas syringae pv. syringae on mango trees is increased by 62-Kb plasmids

被引:0
作者
Cazorla, FM [1 ]
Arrebola, E [1 ]
Abad, C [1 ]
Codina, JC [1 ]
Pérez-García, A [1 ]
De Vicente, A [1 ]
机构
[1] Univ Malaga, Fac Ciencias, Dept Microbiol, Malaga 29071, Spain
来源
PSEUDOMONAS SYRINGAE AND RELATED PATHOGENS: BIOLOGY AND GENETICS | 2003年
关键词
epiphytic fitness; plasmid; copper resistance; ultraviolet light-resistance;
D O I
暂无
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this work, we have studied the relationship between presence of indigenous plasmids and copper- and ultraviolet light-resistance in Pseudomonas syringae pv. syringae strains isolated from mango trees. In such strains, plasmids of 62 kb showed cross-hybridisation with specific sequences of the copper-resistance operon copABCD and the UV-resistance determinant rulAB. Copper-resistance was evaluated by determining the minimal inhibitory concentration (MIC) of copper sulphate, as well as analysing the increase of copper-resistant (Cu-r) bacteria in commercial field plots under copper treatments during three years. UV-resistance was evaluated by performing survival curves of P. s. pv. syringae cells exposed to doses of B+A UV-fractions, as well as the persistence of P. s. pv. syringae strains under field conditions studied on mango leaves directly exposed to solar radiation. Survival curves showed that strains harbouring 62-kb plasmids had a higher survival ability under both conditions. The role of the indigenous plasmids of 62 kb in copper- and UV-resistance was confirmed by evaluating survival of transconjugants obtained by mating assays. The most abundant 62-kb plasmid (62.1) carried copper- and UV-resistance determinants homologous to copABCD and rulAB genes, and was shown to be conjugative and very stable. These results strongly suggest an important contribution of 62-kb plasmids to increasing the epiphytic fitness of P. s. pv. syringae on mango plant surfaces.
引用
收藏
页码:79 / 88
页数:10
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