Human Umbilical Cord-Derived Mesenchymal Stem Cells Improve Learning and Memory Function in Hypoxic-Ischemic Brain-Damaged Rats via an IL-8-Mediated Secretion Mechanism Rather than Differentiation Pattern Induction

被引：49
作者：
Zhou, Xiaoqin [1 ,2 ]
Gu, Jialu [1 ,2 ]
Gu, Yan [1 ,2 ]
He, Mulan [1 ,2 ]
Bi, Yang [3 ]
Chen, Jie [1 ,2 ]
Li, Tingyu [1 ,2 ]
机构：
[1] Chongqing Med Univ, Childrens Hosp, Childrens Nutr Res Ctr, Minist Educ,Key Lab Child Dev & Disorders,Key Lab, Chongqing 400014, Peoples R China
[2] Chongqing Med Univ, Childrens Hosp, Chongqing Engn Res Ctr Stem Cell Therapy, Chongqing, Peoples R China
[3] Chongqing Med Univ, Childrens Hosp, Stem Cell Biol & Therapy Lab, Chongqing, Peoples R China
来源：
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY | 2015年 / 35卷 / 06期
基金：
中国国家自然科学基金;
关键词：
Human umbilical cord-derived mesenchymal stem cells; Hypoxic-ischemic brain damage; IL-8; Learning-memory function; Differentiation; MARROW STROMAL CELLS; CEREBRAL-ISCHEMIA; NEURONAL DIFFERENTIATION; THERAPEUTIC BENEFIT; TRANSPLANTATION; NEUROGENESIS; ANGIOGENESIS; RECOVERY; STROKE;
D O I：
10.1159/000374040
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Background: MSCs are a promising therapeutic resource. Paracrine effects and the induction of differentiation patterns are thought to represent the two primary mechanisms underlying the therapeutic effects of mesenchymal stem cell (MSC) transplantation in vivo. However, it is unclear which mechanism is involved in the therapeutic effects of human umbilical cord-derived MSC (hUC-MSC) transplantation. Methods and Results: Based on flow cytometry analysis, hUC-MSCs exhibited the morphological characteristics and surface markers of MSCs. Following directed neural induction, these cells displayed a neuron-like morphology and expressed high levels of neural markers. All types of hUC-MSCs, including differentiated and redifferentiated cells, promoted learning and memory function recovery in hypoxic-ischemic brain damaged (HIBD) rats. The hUC-MSCs secreted IL-8, which enhanced angiogenesis in the hippocampus via the JNK pathway. However, the differentiated and redifferentiated cells did not exert significantly greater therapeutic effects than the undifferentiated hUC-MSCs. Conclusion: hUC-MSCs display the biological properties and neural differentiation potential of MSCs and provide therapeutic advantages by secreting IL-8, which participates in angiogenesis in the rat HIBD model. These data suggest that hUC-MSC transplantation improves the recovery of neuronal function via an IL-8-mediated secretion mechanism, whereas differentiation pattern induction was limited. Copyright (C) 2015 S. Karger AG, Basel
引用
收藏
页码：2383 / 2401
页数：19
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