Larvicidal Activity of Silver Nanoparticles Synthesized by Pseudomonas fluorescens YPS3 Isolated from the Eastern Ghats of India

Microbial nanoparticles synthesis is a greener approach by which it interconnects the nanotechnology and microbial biotechnology. The present study focussed on the synthesis of AgNP using P. fluorescens YPS3 as a mediated and larvicidal activity of AgNP against Aedes aegypti, Anopheles stephensi and Culex quinquefasciatus mosquito. P. fluorescens-YPS3 mediated AgNPs were characterized by UV-visible spectrophotometer, FT-IR, XRD, FE-SEM, EDX and HR-TEM analysis. The nature of the nanoparticles were confirmed by EDaX and they were polydispersed and spherical in nature with the average size of 26.67 +/- 3nm. The larvicidal activity of the AgNP revealed prominent toxicity on the tested mosquito larvae. AgNPs showed the LC50 value as 80.658g/ml, 94.225g/ml, and 113.238g/ml A. aegypti, A. stephensi and C. quinquefasciatus respectively. P. fluorescensYPS3 synthesized nanoparticles found to have prominent bioactivity on mosquito larvae and thus can be used as biocontrol agent to control mosquito population.