Achromobacter marplatensis sp nov., isolated from a pentachlorophenol-contaminated soil

被引:40
作者
Gomila, Margarita [1 ,2 ]
Tvrzova, Ludmila [3 ]
Teshim, Andrea [3 ]
Sedlacek, Ivo [4 ]
Gonzalez-Escalona, Narjol [5 ]
Zdrahal, Zbynek [6 ]
Sedo, Ondrej [6 ]
Froilan Gonzalez, Jorge [7 ]
Bennasar, Antonio [1 ,2 ,8 ]
Moore, Edward R. B. [9 ,10 ]
Lalucat, Jorge [1 ,2 ]
Murialdo, Silvia E. [7 ]
机构
[1] Univ Illes Balears, Dept Biol, Palma De Mallorca 07122, Illes Balears, Spain
[2] Inst Mediterrani Estudis Avancats CSIC UIB, Palma De Mallorca 07122, Illes Balears, Spain
[3] Masaryk Univ, Fac Sci, Dept Expt Biol, Div Microbiol, Brno 60200, Czech Republic
[4] Masaryk Univ, Fac Sci, Dept Expt Biol, Czech Collect Microorganisms, Brno 60200, Czech Republic
[5] US FDA, Ctr Food Safety & Appl Nutr, College Pk, MD USA
[6] Masaryk Univ, Fac Sci, Dept Expt Biol, Div Funct Genom & Prote, Brno 62500, Czech Republic
[7] Univ Nacl Mar del Plata, RA-7600 Buenos Aires, Argentina
[8] Inst Univ Invest Ciencies Salut IUNICS UIB, Palma De Mallorca 07122, Spain
[9] Sahlgrens Univ Hosp, CCUG, Dept Clin Bacteriol, Gothenburg, Sweden
[10] Univ Gothenburg, Sahlgrenska Acad, Gothenburg, Sweden
关键词
HUMAN CLINICAL-SAMPLES; DENITRIFICANS RUGER; RECLASSIFICATION; PIECHAUDII;
D O I
10.1099/ijs.0.025304-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A polyphasic taxonomic approach was applied to the study of a Gram-negative bacterium (B2(T)) isolated from soil by selective enrichment with pentachlorophenol. 16S rRNA gene sequence analysis of strain B2(T) showed that the strain belongs to the genus Achromobacter within the Betaproteobacteria. The 16S rRNA gene sequence displayed more than 99% similarity to the sequences of the type strains of all species of Achromobacter, with the highest sequence similarity to those of Achromobacter spanius CCM 7183(T) and A. piechaudii CCM 2986(T) (99.8%). On the basis of phylogenetic analysis, genomic DNA-DNA relatedness and phenotypic characteristics, including chemotaxonomic (cellular fatty acid profile) analysis, a novel species is proposed, Achromobacter marplatensis sp. nov., with the type strain B2(T) (=CCM 7608(T) =CCUG 56371(T) =CECT 7342(T)).
引用
收藏
页码:2231 / 2237
页数:7
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