The adult microbiome of healthy and otitis patients: Definition of the core healthy and diseased ear microbiomes

被引:18
作者
Burton, Maria [1 ]
Krumbeck, Janina A. [1 ]
Wu, Guangxi [1 ]
Tang, Shuiquan [1 ]
Prem, Aishani [1 ]
Gupta, Aditya K. [2 ,3 ]
Dawson, Thomas L., Jr. [4 ,5 ]
机构
[1] Zymo Res Corp, Irvine, CA USA
[2] Mediprobe Res Inc, London, ON, Canada
[3] Univ Toronto, Toronto, ON, Canada
[4] Skin Res Inst, Singapore, Singapore
[5] Med Univ South Carolina, Coll Pharm, Dept Drug Discovery, Charleston, SC 29425 USA
关键词
SEBORRHEIC DERMATITIS; CANDIDA-ORTHOPSILOSIS; METAPSILOSIS; MEDIA;
D O I
10.1371/journal.pone.0262806
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Otitis media (OM) and externa (OE) are painful, recurrent ear conditions. As most otitis publications focus on the bacterial content of childhood ears, there remains a dearth of information regarding the adult ear microbiome including both bacteria and fungi. This study compares the outer ear microbiome of healthy adults to adults affected by OE and OM using both intergenic-transcribed-spacer (ITS) and 16S-rDNA sequencing. The adult ear core microbiome consists of the prokaryote Cutibacterium acnes and the eukaryotic Malassezia arunalokei, M. globosa, and M. restricta. The healthy ear mycobiome is dominated by Malassezia and can be divided into two groups, one dominated by M. arunalokei, the other by M. restricta. Microbiome diversity and biomass varied significantly between healthy and diseased ears, and analyses reveal the presence of a potential mutualistic, protective effect of Malassezia species and C. acnes. The healthy ear core microbiome includes the bacteria Staphylococcus capitis and S. capitis/caprae, while the diseased ear core is composed of known bacterial and fungal pathogens including Aspergillus sp., Candida sp., Pseudomonas aeruginosa, S. aureus, and Corynebacterium jeikeium. The data presented highlight the need for early detection of the cause of otitis to direct more appropriate, efficient treatments. This will improve patient outcomes and promote improved antimicrobial stewardship.
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页数:18
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