Facile preparation of carbohydrate microarrays by site-specific, covalent immobilization of unmodified carbohydrates on hydrazide-coated glass slides

被引:144
|
作者
Lee, M [1 ]
Shin, I [1 ]
机构
[1] Yonsei Univ, Dept Chem, Seoul 120749, South Korea
关键词
D O I
10.1021/ol051753z
中图分类号
O62 [有机化学];
学科分类号
070303 ; 081704 ;
摘要
A new, simple and efficient immobilization method to attach mono-, di-, oligo-, and polysaccharides to hydrazide-coated glass slides was developed. Protein and cell-binding experiments show that the carbohydrate microarrays prepared by this method are applicable for the rapid analysis of protein-carbohydrate interactions and fast detection of pathogens.
引用
收藏
页码:4269 / 4272
页数:4
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