Transglycosylation of thiamin by fungal β-N-acetylhexosaminidases

被引:7
作者
Kren, V
Hunkova, Z
Halada, P
Suzuki, Y
机构
[1] Acad Sci Czech Republ, Inst Microbiol, Lab Biotransformat, CZ-14220 Prague 4, Czech Republic
[2] Okayama Univ, Bioresources Res Inst, Okayama 7100046, Japan
关键词
thiamin; vitamin; beta-N-acetylhexosaminidases; transglycosylation; Aspergillus oryzae;
D O I
10.1271/bbb.62.2415
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A new thiamin glycoside, e.g., thiamin beta-D-2-deoxy-2-acetamidoglucopyranoside was prepared by transglycosylation by beta-N-acetylhexosaminidase from Aspergillus oryzae and characterized spectrally. Series of other fungal beta-N-acetyl-hexosaminidases from A. awamori, A. tamari, A. terreus, and Penicillium oxalicum were shown to be able to synthesize the same compound in lower yields.
引用
收藏
页码:2415 / 2417
页数:3
相关论文
共 50 条
  • [41] Enzymatic synthesis of a neoglycoconjugate by transglycosylation with Arthrobacter endo-β-N-acetylglucosaminidase:: A substrate for colorimetric detection of endo-β-acetylglucosaminidase activity
    Takegawa, K
    Fujita, K
    Fan, JQ
    Tabuchi, M
    Tanaka, N
    Kondo, A
    Iwamoto, H
    Kato, I
    Lee, YC
    Iwahara, S
    ANALYTICAL BIOCHEMISTRY, 1998, 257 (02) : 218 - 223
  • [42] Characterization of endo-α-N-acetylgalactosaminidase from Bacillus sp and syntheses of neo-oligosaccharides using its transglycosylation activity
    Ashida, H
    Yamamoto, K
    Murata, T
    Usui, T
    Kumagai, H
    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2000, 373 (02) : 394 - 400
  • [43] Secondary structure by circular dichroismspectroscopy of β-N-acetylglucosaminidase from Lecanicillium lecanii and its relationship with hydrolytic and transglycosylation activities at different pH values
    Rojas-Osnaya, J.
    Tello-Solis, S. R.
    Shirai, K.
    REVISTA MEXICANA DE INGENIERIA QUIMICA, 2024, 23 (01):
  • [44] Kinetic studies on endo-β-galactosidase by a novel colorimetric assay and synthesis of N-acetyllactosamine-repeating oligosaccharide β-glycosides using its transglycosylation activity
    Murata, T
    Hattori, T
    Amarume, S
    Koichi, A
    Usui, T
    EUROPEAN JOURNAL OF BIOCHEMISTRY, 2003, 270 (18): : 3709 - 3719
  • [45] Arthrobacter Endo-β-N-Acetylglucosaminidase Shows Transglycosylation Activity on Complex-Type N-Glycan Oxazolines: One-Pot Conversion of Ribonuclease B to Sialylated Ribonuclease C
    Huang, Wei
    Yang, Qiang
    Umekawa, Midori
    Yamamoto, Kenji
    Wang, Lai-Xi
    CHEMBIOCHEM, 2010, 11 (10) : 1350 - 1355
  • [46] Transglycosylation behavior of Mucor hiemalis endo-β-N-acetylglucosaminidase to β-cyclodextrin derivatives with multivalent glucose moieties for synthesizing cyclodextrin-based oligosaccharide clusters
    Tomabechi, Yusuke
    Oda, Yoshiki
    Yamamoto, Kenji
    Yamanoi, Takashi
    CARBOHYDRATE RESEARCH, 2025, 548
  • [47] Synthesis of Chitin Oligosaccharides Using Dried Stenotrophomonas maltophilia Cells Containing a Transglycosylation Reaction-Catalyzing β-N-Acetylhexosaminidase as a Whole-Cell Catalyst
    Asaki Uehara
    Narumi Takahashi
    Mei Moriyama
    Takako Hirano
    Wataru Hakamata
    Toshiyuki Nishio
    Applied Biochemistry and Biotechnology, 2018, 184 : 673 - 684
  • [48] Transglycosylation reaction of Mucor hiemalis endo-β-N-acetylglucosaminidase using sugar derivatives modified at C-1 or C-2 as oligosaccharide acceptors
    Yamanoi, T
    Tsutsumida, M
    Oda, Y
    Akaike, E
    Osumi, K
    Yamamoto, K
    Fujita, K
    CARBOHYDRATE RESEARCH, 2004, 339 (07) : 1403 - 1406
  • [49] Sequencing, cloning and high-yield expression of a fungal β-N-acetylhexosaminidase in Pichia pastoris
    Slamova, Kristyna
    Bojarova, Pavla
    Gerstorferova, Daniela
    Fliedrova, Barbora
    Hofmeisterova, Jana
    Fiala, Martin
    Pompach, Petr
    Kren, Vladimir
    PROTEIN EXPRESSION AND PURIFICATION, 2012, 82 (01) : 212 - 217
  • [50] Synthesis of p-nitrophenyl sulfated disaccharides with β-D-(6-sulfo)-GlcNAc units using β-N-acetylhexosaminidase from Aspergillus oryzae in a transglycosylation reaction
    Zeng, Xiaoxiong
    Sun, Yi
    Ye, Hong
    Liu, Jun
    Uzawa, Hirotaka
    BIOTECHNOLOGY LETTERS, 2007, 29 (07) : 1105 - 1110