Effect of Various Factors on Orange II Decoloration Reaction by Horseradish Peroxidase in the Presence of Detergent Enzymes

被引:0
作者
Morita, Miyuki [1 ]
Tani, Michiko [1 ]
Onikoshi, Mayuko [1 ]
Mase, Kiyomi [2 ]
Masuko, Fumi [3 ]
机构
[1] Hokkaido Univ, Kita Ku, 5-3-1 Ainosato, Sapporo, Hokkaido, Japan
[2] Nagoya Womens Univ, Mizuho Ku, 3-40 Shioji Cho, Nagoya, Aichi 4678610, Japan
[3] Japan Womens Univ, Bunkyo Ku, 2-8-1 Mejirodai, Tokyo 1128681, Japan
关键词
horseradish peroxidase; Orange II; detergent enzymes; Ca and Mg ion; decoloration reaction; HYDROGEN-PEROXIDE; LIPASE; ION;
D O I
10.5650/jos.ess17103
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
The effects of four detergent enzymes on the Orange II decoloration reaction by horseradish peroxidase (HRP) were investigated. Stainzyme, Lipex, Celluclean, and Savinase did not affect the decoloration reaction up to a concentration of 0.1% Under weak alkaline to alkaline conditions, the presence of four enzymes did not affect the decoloration reaction, but affected the decoloration reaction under neutral to acidic conditions. The effect of Ca2+ and Mg2+ concentrations on Orange II decoloration reactions in the presence of four enzymes was also investigated. Ca2+ and Mg2+ did not affect the decoloration reaction up to 500 ppm.
引用
收藏
页码:315 / 319
页数:5
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