Distribution and characterization of immunoreactive prolactin-releasing peptide (PrRP) in rat tissue and plasma

被引：64

作者：

Matsumoto, H ^{[1
]}

Murakami, Y ^{[1
]}

Horikoshi, Y ^{[1
]}

Noguchi, J ^{[1
]}

Habata, Y ^{[1
]}

Kitada, C ^{[1
]}

Hinuma, S ^{[1
]}

Onda, H ^{[1
]}

Fujino, M ^{[1
]}

机构：

[1] Takeda Chem Ind Ltd, Div Pharmaceut Discovery, Discovery Res Labs 1, Ibaraki, Osaka 3004293, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1999年 / 257卷 / 02期

关键词：

D O I：

10.1006/bbrc.1999.0463

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We established a sensitive and specific two-site enzyme immunoassay (EIA) for prolactin-releasing peptide (PrRP) using two region-specific monoclonal antibodies. We investigated the tissue distribution and the plasma concentration of immunoreactive (ir-) PrRP in rats using this assay. Ir-PrRP was widely distributed in the central nervous system and pituitary gland. The highest concentration of ir-PrRP was found in the hypothalamus. In peripheral tissues, appreciable levels of ir-PrRP were found only in the adrenal gland. The mean plasma concentration of ir-PrRP was 0.13 +/- 0.01 fmol/ml (mean +/- SEM). In reverse-phase and gel-filtration high performance liquid chromatography, hypothalamic ir-PrRP eluted at a position identical to that of PrRP31 and PrRP20. On the other hand, ir-PrRP from the adrenal gland and plasma eluted only at the position of synthetic PrRP31, indicating that molecular forms of ir-PrRP in vivo differed among tissues. (C) 1999 Academic Press.

引用

页码：264 / 268

页数：5

共 8 条

[1]

FUJII R, 1999, UNPUB REG PEPTIDE

[2] A prolactin-releasing peptide in the brain [J].

Hinuma, S ;

Habata, Y ;

Fujii, R ;

Kawamata, Y ;

Hosoya, N ;

Fukusumi, S ;

Kitada, C ;

Masuo, Y ;

Asano, T ;

Matsumoto, H ;

Sekiguchi, M ;

Kurokawa, T ;

Nishimura, O ;

Onda, H ;

Fujino, M .

NATURE, 1998, 393 (6682) :272-276

[3] MONOCLONAL-ANTIBODIES TO HUMAN INTERFERON-GAMMA .2. ANTIBODIES WITH NEUTRALIZING ACTIVITY [J].

ICHIMORI, Y ;

SUZUKI, N ;

KITADA, C ;

TSUKAMOTO, K .

HYBRIDOMA, 1987, 6 (02) :173-181

[4]

MARUYAMA S, 1999, IN PRESS ENDOCRINOLO

[5] ABUNDANCE OF ENDOTHELIN-3 IN RAT INTESTINE, PITUITARY-GLAND AND BRAIN [J].

MATSUMOTO, H ;

SUZUKI, N ;

ONDA, H ;

FUJINO, M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 164 (01) :74-80

[6] A SENSITIVE SANDWICH-ENZYME IMMUNOASSAY FOR HUMAN ENDOTHELIN [J].

SUZUKI, N ;

MATSUMOTO, H ;

KITADA, C ;

MASAKI, T ;

FUJINO, M .

JOURNAL OF IMMUNOLOGICAL METHODS, 1989, 118 (02) :245-250

[7] PRODUCTION OF ENDOTHELIN-1 AND BIG-ENDOTHELIN-1 BY TUMOR-CELLS WITH EPITHELIAL-LIKE MORPHOLOGY [J].

SUZUKI, N ;

MATSUMOTO, H ;

KITADA, C ;

KIMURA, S ;

FUJINO, M .

JOURNAL OF BIOCHEMISTRY, 1989, 106 (05) :736-741

[8] PACAP STIMULATES CATECHOLAMINE RELEASE FROM ADRENAL-MEDULLA - A NOVEL NONCHOLINERGIC SECRETAGOGUE [J].

WATANABE, T ;

SHIMAMOTO, N ;

TAKAHASHI, A ;

FUJINO, M .

AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 1995, 269 (05) :E903-E909

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