An accurate and sensitive Coomassie Brilliant Blue G-250-based assay for protein determination

被引：165

作者：

Grintzalis, Konstantinos ^{[1
]}

Georgiou, Christos D. ^{[2
]}

Schneider, Yves-Jacques ^{[1
]}

机构：

[1] UCL, Inst Life Sci, Lab Cellular Nutr & Toxicol Biochem, Louvain La Neuve, Belgium

[2] Univ Patras, Dept Biol, Sect Genet Cell Biol & Dev, Patras, Greece

来源：

ANALYTICAL BIOCHEMISTRY | 2015年 / 480卷

关键词：

Protein quantification; Bradford; Coomassie Brilliant Blue G (CBB); QUANTITIES; BINDING;

D O I：

10.1016/j.ab.2015.03.024

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this protocol we present a rapid and sensitive assay for the accurate determination of protein concentration. The assay is a modification of a previous method, and measures minimum 0.21 mu g protein. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：28 / 30

页数：3

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