A gluten metabolism study in healthy individuals shows the presence of faecal glutenasic activity

被引:25
作者
Caminero, Alberto [1 ,6 ]
Nistal, Esther [6 ]
Arias, Laura [3 ]
Vivas, Santiago [3 ]
Comino, Isabel [4 ]
Real, Ana [4 ]
Sousa, Carolina [4 ]
Ruiz de Morales, Jose M. [2 ]
Ferrero, Miguel A. [1 ,5 ]
Rodriguez-Aparicio, Leandro B. [1 ,5 ]
Casqueiro, Javier [1 ,6 ]
机构
[1] Univ Leon, Inst Biol Mol Genom & Prote INBIOMIC, E-24071 Leon, Spain
[2] Hosp Univ Leon, Dept Inmunol, Leon 24071, Spain
[3] Hosp Univ Leon, Dept Gastroenterol, Leon 24071, Spain
[4] Univ Seville, Fac Farm, Dept Microbiol & Parasitol, Seville 410120, Spain
[5] Univ Leon, Area Bioquim, Fac Biol & Ciencias Ambientales, E-24071 Leon, Spain
[6] Univ Leon, Area Microbiol, E-24071 Leon, Spain
关键词
Gluten; Coeliac disease; Faecal glutenasic activity; Gut microbiota; CHAIN FATTY-ACIDS; CELIAC-DISEASE; RESISTANT PEPTIDES; HUMAN COLON; FERMENTATION; RATS; MICROBIOTA; FRACTIONS; CHILDREN; HUMANS;
D O I
10.1007/s00394-011-0214-3
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
To study the gluten metabolism in healthy individuals and its effect over the intestinal microbial activity. The faeces of eleven healthy subjects were analysed under 4 diet regimens: their normal gluten diet, a strict gluten-free diet (GFD), a GFD with a supplemental intake of 9 g gluten/day and a GFD with a supplemental intake of 30 g gluten/day. Gluten content, faecal tryptic activity (FTA), short-chain fatty acids (SCFAs) and faecal glutenasic activity (FGA) were analysed in faecal samples. Faecal gluten contents, FTA, SCFAs and FGA varied significantly with different levels of gluten intake in the diet. When high gluten doses (30 g/day) were administered in the diet, SCFA concentrations (70.5 mmoles/kg faeces) were significantly different from those from the GFD period (33.8 mmoles/kg faeces) of the experiment. However, the FTA showed significant differences between the GFD (34 units) and the normal gluten-containing diet (60 units) and also between the GFD and the GFD + 30 g of gluten/day (67 units). When gluten was present in the diet, gluten was detected in the faeces, showing that at least a portion of the gluten ingested is eliminated in the large intestine, providing a substrate for intestinal microbial proteases. We have also shown the presence of faecal glutenasic activity that increased proportionally with the gluten intake in the diet, showing an enzymatic activity of 993 units in DSG, 2,063 units in DSG + 9 g and 6,090 units in DSG + 30 g. The activity of the intestinal microbiota is modified by gluten intake in the diet. The incorporation of gluten in the diet increases the activity of a gluten proteolytic activity in the faeces.
引用
收藏
页码:293 / 299
页数:7
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