Dark-colored maple syrup treatment induces S-phase cell cycle arrest via reduced proliferating cell nuclear antigen expression in colorectal cancer cells

被引:6
作者
Yamamoto, Tetsushi [1 ]
Nishita, Tomoyo [1 ]
Taga, Atsushi [1 ]
机构
[1] Kindai Univ, Sch Pharm, Pathol & Biomol Anal Lab, 3-4-1 Kowakae, Higashiosaka, Osaka 5778502, Japan
关键词
maple syrup; colorectal cancer; proteomics; cell cycle; proliferating cell nuclear antigen; TOKUSHIMA FATTY RATS; BREAST-CANCER; DAIRY-PRODUCTS; PLASMA-GLUCOSE; RISK; FOODS; PCNA; CALCIUM; SAP; IDENTIFICATION;
D O I
10.3892/ol.2019.9928
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Maple syrup is a natural sweetener that is consumed worldwide. It has been previously reported that dark-colored maple syrup exerts an inhibitory effect on colorectal cancer (CRC) proliferation and invasion. In the present study, the underlying mechanism of CRC cell growth inhibition was examined with dark-colored maple syrup treatment using a shotgun liquid chromatography-tandem mass spectrometry-based global proteomic approach. Applying a semi-quantitative method based on spectral counting, 388 proteins were identified with expression changes of >1.5-fold following dark-colored maple syrup treatment. Gene Ontology analysis revealed that these proteins possessed cell cycle-associated functions. It was also indicated that CRC cells treated with dark-colored maple syrup exhibited decreased proliferating cell nuclear antigen (PCNA) expression and S-phase cell cycle arrest. Dark-colored maple syrup treatment also resulted in altered expression of cell cycle-associated genes, including cyclin-dependent kinase (CDK)4 and CDK6. In conclusion, these data suggested that dark-colored maple syrup induced S-phase cell cycle arrest in CRC cells by reducing the expression of PCNA and regulating cell cycle-associated genes. These findings suggest that dark-colored maple syrup may be a source of compounds for the development of novel drugs for colorectal cancer treatment.
引用
收藏
页码:2713 / 2720
页数:8
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