Detection and quantification of Escherichia coli and Pseudomonas aeruginosa in cow milk by near-infrared spectroscopy

被引:24
作者
Camara-Martos, Fernando [1 ]
Almeida Lopes, Joao [2 ]
Moreno-Rojas, Rafael [1 ]
Perez-Rodriguez, Fernando [1 ,3 ]
机构
[1] Univ Cordoba, Dept Bromatol & Tecnol Alimentos, Cordoba 14014, Spain
[2] Univ Porto, REQUIMTE, Serv Quim Fis, Fac Farm, P-4099030 Oporto, Portugal
[3] Optimum Qual Pol Tecnocordoba, Cordoba 14014, Spain
关键词
NIR; Milk; Chemometrics; Escherichia coli; Pseudomonas aeruginosa; PLSDA; AEROBIC-BACTERIA COUNT; IDENTIFICATION; SPOILAGE; QUALITY; NIR; COMPONENTS; SPECTRA; SYSTEM; SKIM;
D O I
10.1111/1471-0307.12191
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
This work investigated the potential of NIR technology to be applied in the dairy industry for the detection of micro-organisms. To this end, two types of cow milk samples were studied, one in which only bacterial biomass was considered and the other in which bacteria were cultured and grown in milk for 24h. The study was carried out using two micro-organisms Escherichia coli and Pseudomonas aeruginosa. Both types of samples with different counts of both micro-organisms were analysed by a NIR analyser in the range 10000-4000cm(-1) based on transmittance measurements. Multivariate models indicated that a better discrimination between micro-organisms was attained in those milk samples in which micro-organisms have been grown.
引用
收藏
页码:357 / 365
页数:9
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